Related Experiment Video

Updated: Sep 3, 2025

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
08:04

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

19.1K

TSPAN8 alleviates high glucose-induced apoptosis and autophagy via targeting mTORC2

Langen Zhuang1, Guoxi Jin1, Xiaolei Hu1

  • 1Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.

Cell Biology International
|July 29, 2022
PubMed

Insights

Tetraspanin 8 (TSPAN8) mitigates high glucose-induced cell damage in diabetic nephropathy. Overexpression of TSPAN8 reduces cell death and autophagy, suggesting its potential as a therapeutic target for diabetic kidney disease.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Nephrology

Background:

  • Tetraspanin 8 (TSPAN8) plays roles in cell signaling and development.
  • Its involvement in diabetic nephropathy (DN) progression is not well understood.

Purpose of the Study:

  • To investigate the role of TSPAN8 in high glucose (HG)-induced autophagy and apoptosis in HK-2 cells.
  • To explore TSPAN8's potential as a therapeutic target for DN.

Main Methods:

  • RT-PCR and Western Blot to measure TSPAN8 levels.
  • CCK-8 assay for cell proliferation and flow cytometry for apoptosis.
  • TSPAN8 gene transfection to evaluate functional roles.

Main Results:

  • TSPAN8 levels were decreased in DN patients' blood and HG-induced HK-2 cells.
  • TSPAN8 overexpression protected HK-2 cells from HG-induced apoptosis.
  • TSPAN8 formed a complex with Rictor and mTORC2, suppressing HG-induced autophagy via mTOR activity.

Conclusions:

  • TSPAN8 mitigates high glucose-induced autophagy and apoptosis in renal cells.
  • TSPAN8 represents a potential therapeutic target for diabetic nephropathy treatment.
Keywords:
TSPAN8apoptosisautophagydiabetic nephropathy (DN)mTORC2

More Related Videos

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

8.7K
Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen19:44

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

18.8K

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.9K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.2K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.4K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.8K