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Related Experiment Videos

Canine parvovirus serology: a collaborative assay.

P R Luff, G W Wood, C N Hebert

    The Veterinary Record
    |March 21, 1987
    PubMed
    Summary

    This study assessed canine parvovirus antibody testing across 15 labs. Results showed variability, but a standard antiserum helped, with vaccine manufacturers showing less variation in canine parvovirus serology.

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    Area of Science:

    • Veterinary immunology
    • Canine infectious diseases

    Background:

    • Canine parvovirus (CPV) poses a significant threat to canine health.
    • Accurate serological testing is crucial for diagnosing CPV infections and evaluating vaccine efficacy.
    • Standardization of CPV antibody detection methods is needed.

    Purpose of the Study:

    • To evaluate the variability in canine parvovirus antibody testing among different laboratories.
    • To assess the utility of a potential British standard canine parvovirus antiserum.
    • To compare results from diagnostic laboratories and vaccine manufacturers.

    Main Methods:

    • Coded canine serum samples were distributed to 15 laboratories for antibody testing against canine parvoviruses.
    • Laboratories employed various methods including haemagglutination inhibition (HAI), enzyme-linked immunosorbent assay (ELISA), and serum neutralisation tests.
    • One serum sample was designated as a potential British standard canine parvovirus antiserum.

    Main Results:

    • Adequate analysis was feasible primarily for haemagglutination inhibition test results.
    • The use of a standard serum partially reduced inter-laboratory variability.
    • Significant residual variability was attributed to specific laboratories, with vaccine manufacturers demonstrating less variability than diagnostic labs.

    Conclusions:

    • Inter-laboratory variation in canine parvovirus antibody testing is a concern.
    • A standard canine parvovirus antiserum can help mitigate some variability.
    • Further standardization efforts are warranted to improve consistency in canine parvovirus serology.

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