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Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Hematopoiesis01:21

Hematopoiesis

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The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
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Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Lineage Commitment01:21

Lineage Commitment

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Commitment is the  process whereby stem cells:
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Production of Formed Elements01:34

Production of Formed Elements

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Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
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Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

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Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
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Related Experiment Video

Updated: Sep 3, 2025

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
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Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

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Endothelial-specific Gata3 expression is required for hematopoietic stem cell generation.

Nada Zaidan1, Leslie Nitsche2, Evangelia Diamanti3

  • 1Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh EH16 4UU, UK; Department of Haematology, Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 0AW, UK.

Stem Cell Reports
|July 29, 2022
PubMed
Summary

GATA3 is crucial for hematopoietic stem cell (HSC) development during the endothelial-to-hematopoietic transition (EHT). Its absence prevents hemogenic endothelial cells from maturing into HSCs, highlighting its core regulatory role.

Keywords:
Cdkn1cGata3aorta-gonads-mesonephroscell cycleendothelial-to-hematopoietic transitionhematopoietic stem cellhemogenic endothelial cellsp57Kip2

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Last Updated: Sep 3, 2025

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Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
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Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Published on: May 8, 2018

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Area of Science:

  • Developmental biology
  • Hematopoiesis
  • Stem cell biology

Background:

  • Generating transplantable hematopoietic stem cells (HSCs) in vitro requires understanding in vivo development.
  • The endothelial-to-hematopoietic transition (EHT) is a complex process involving precise gene regulation and microenvironmental signals.
  • GATA3 was previously linked to HSC support in specific niches.

Purpose of the Study:

  • To investigate the cell-intrinsic role of GATA3 in the endothelial-to-hematopoietic transition (EHT).
  • To determine if GATA3 is functionally required for HSC maturation during EHT.

Main Methods:

  • Analysis of GATA3 expression in hemogenic endothelial cells and early HSC precursors.
  • Endothelial-specific deletion of the Gata3 gene in vivo.
  • Assessment of HSC maturation following Gata3 deletion.

Main Results:

  • GATA3 is expressed in hemogenic endothelial cells and early HSC precursors.
  • GATA3 expression correlates with a more quiescent state in these cells.
  • Endothelial-specific Gata3 deletion impairs the maturation of hemogenic endothelial cells into HSCs.

Conclusions:

  • GATA3 plays a cell-intrinsic role in regulating the endothelial-to-hematopoietic transition (EHT).
  • GATA3 is functionally essential for the maturation of endothelial cells into hematopoietic stem cells (HSCs).
  • GATA3 is a core component of the regulatory network governing EHT and HSC production.