Effect of a GLP-1 mimetic on the insulin response to oral sugar testing in horses

Insulin dysregulation (ID) is the most important risk factor for the development of laminitis in horses and therapies to control it are needed.

To assess the effects of a single dose of the synthetic GLP-1 analog exenatide on postprandial insulin dynamics. We hypothesized that exenatide would improve insulin sensitivity and lower postprandial blood insulin concentrations.

Randomized, crossover, experimental study.

Six horses (3 mares, 3 geldings; 2 with normal insulin regulation [NIR] and 4 with mild ID).

Horses completed both study arms: subcutaneous administration of exenatide (or no treatment) 30 min before an oral sugar test (0.15 ml/kg of Karo Syrup). Blood samples obtained over 240 min were assayed for glucose, insulin, lactate, c-peptide and total GLP-1. The area under the curve (AUC) was calculated using the trapezoidal rule. Insulin sensitivity (S_I) was estimated using a mathematical model.

Exenatide resulted in a postprandial decrease of 20% (effect size: 2673 µU·min/ml; 95% CI: 900 - 4446 µU·min/ml; P = 0.003) in AUC of plasma insulin (control; mean AUC insulin: 11,989 µU·min/ml; 95% CI: 9673 - 14,305 µU·min/ml, exenatide; mean AUC insulin: 9316 µU·min/ml; 95% CI: 7430 - 11,202 µU·min/ml). Exenatide resulted in an approximately threefold increase (effect size: 5.56 10^-4· µU/ml^-1·min^-1; 95% CI: 0.95 - 10.1 10^-4· µU/ml^-1·min^-1; P = 0.02) in estimated insulin sensitivity (control mean S_I: 1.93 10^-4· µU/ml^-1·min^-1; 95% CI: 0.005 - 3.86 10^-4·µU/ml^-1·min^-1 vs. exenatide mean S_I: 7.49 10^-4· µU/ml^-1·min^-1; 95% CI: 3.46 - 11.52 10^-4· µU/ml^-1·min^-1).

The decrease in insulin response to carbohydrates was due to an increase in whole-body insulin sensitivity. GLP-1 agonists may have therapeutic potential for ID in horses.