Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Kendall's Coefficient of Concordance01:20

Kendall's Coefficient of Concordance

506
Kendall's Coefficient of Concordance (W), also known as Kendall's W, is a non-parametric statistical measure used to assess the agreement or concordance between multiple raters or judges when they rank a set of items. It is often used when you have ordinal data (ranks) and you want to see if there is consistency or consensus among the raters. It is widely applied in research areas such as psychology, medicine, and social sciences, where multiple judges are asked to rank or rate subjects...
506
McNemar's Test01:23

McNemar's Test

397
McNemar's Test is a nonparametric statistical test used to determine if there is a significant difference in proportions between two related groups when the outcome is binary (e.g., yes/no, success/failure). It is beneficial when we have paired data, such as pre-test/post-test designs, where the same subjects are measured under two different conditions. The test is named after the statistician Quinn McNemar, who introduced it in 1947. It is commonly used in situations where subjects are...
397
Accuracy and Errors in Hypothesis Testing01:13

Accuracy and Errors in Hypothesis Testing

280
Hypothesis testing is a fundamental statistical tool that begins with the assumption that the null hypothesis H0 is true. During this process, two types of errors can occur: Type I and Type II. A Type I error refers to the incorrect rejection of a true null hypothesis, while a Type II error involves the failure to reject a false null hypothesis.
In hypothesis testing, the probability of making a Type I error, denoted as α, is commonly set at 0.05. This significance level indicates a 5%...
280
Friedman Two-way Analysis of Variance by Ranks01:21

Friedman Two-way Analysis of Variance by Ranks

287
Friedman's Two-Way Analysis of Variance by Ranks is a nonparametric test designed to identify differences across multiple test attempts when traditional assumptions of normality and equal variances do not apply. Unlike conventional ANOVA, which requires normally distributed data with equal variances, Friedman's test is ideal for ordinal or non-normally distributed data, making it particularly useful for analyzing dependent samples, such as matched subjects over time or repeated measures...
287
Cochran's Q Test01:17

Cochran's Q Test

526
Cochran's Q Test is a nonparametric statistical test used to determine if there are potential differences in the outcomes of three or more related groups on a binary (yes/no) or dichotomous outcome. It is essentially an extension of the McNemar Test, which is limited to two related samples - Cochran's Q test can handle three or more related samples, making it more versatile in scenarios where subjects are measured under multiple conditions. The test statistic follows a Chi-Square...
526
Decision Making: Traditional Method01:14

Decision Making: Traditional Method

4.1K
The process of hypothesis testing based on the traditional method includes calculating the critical value, testing the value of the test statistic using the sample data, and interpreting these values.
First, a specific claim about the population parameter is decided based on the research question and is stated in a simple form. Further, an opposing statement to this claim is also stated. These statements can act as null and alternative hypotheses, out of which a null hypothesis would be a...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gab1 but not Grb2 mediates tumor progression in Met overexpressing colorectal cancer cells.

Carcinogenesis·2008
Same author

Long-term donor-specific tolerance in rat cardiac allografts by intrabone marrow injection of donor bone marrow cells.

Transplantation·2008
Same author

Lsr2 of Mycobacterium tuberculosis is a DNA-bridging protein.

Nucleic acids research·2008
Same author

Amphetamine selectively enhances avoidance responding to a less salient stimulus in rats.

Journal of neural transmission (Vienna, Austria : 1996)·2008
Same author

Retrospective analysis of anterior correction and fusion for adolescent idiopathic thoracolumbar/lumbar scoliosis: the relationship between preserving mobile segments and trunk balance.

International orthopaedics·2008
Same author

Intrarenal antigens activate CD4+ cells via co-stimulatory signals from dendritic cells.

Journal of the American Society of Nephrology : JASN·2008
Same journal

A novel activation-related gene P158 is essential for evagination and early development of Echinococcus multilocularis protoscoleces.

Parasites & vectors·2026
Same journal

Seroprevalence of Dirofilaria immitis in traveling dogs: implications for transboundary pathogen movement.

Parasites & vectors·2026
Same journal

A comprehensive proteome and the first phosphoproteome reveal extensive phosphorylation of carbohydrate metabolism in Cryptosporidium parvum sporozoites.

Parasites & vectors·2026
Same journal

Molecular characterization of Blastocystis sp., Cryptosporidium spp., and Giardia spp. in Eld's deer (Rucervus eldii) and their forest rangers in Hainan, China.

Parasites & vectors·2026
Same journal

Droplet digital PCR-based pathogen monitoring system improves identifying Haemaphysalis longicornis infected with Bandavirus dabieense and other tick-borne pathogens at low concentrations.

Parasites & vectors·2026
Same journal

Field evaluation of a novaluron-based larvicide tablet (MOSQINOk<sup>®</sup> 0.8P) in irrigated rice agroecosystems of The Gambia: a controlled quasi-experimental study.

Parasites & vectors·2026
See all related articles

Related Experiment Video

Updated: Sep 3, 2025

Comparative Analysis of Automatic Fecal Analyzer versus Direct Wet Smear Microscopy for Detecting Parasitic Infections in Stool Samples
04:57

Comparative Analysis of Automatic Fecal Analyzer versus Direct Wet Smear Microscopy for Detecting Parasitic Infections in Stool Samples

Published on: April 25, 2025

579

Methodological issues on evaluating agreement between two detection methods by Cohen's kappa analysis.

Ming Li1, Tianfei Yu2

  • 1College of Computer and Control Engineering, Qiqihar University, Qiqihar, 161006, China.

Parasites & Vectors
|July 29, 2022
PubMed
Summary
This summary is machine-generated.

This study questions the statistical agreement between a PCR method and ELISA for detecting Plasmodium vivax in mosquitoes. Improved statistical analysis is recommended for reliable malaria research findings.

Keywords:
COX-I PCRCSP ELISACohen’s kappaMosquitoesPlasmodium

More Related Videos

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
05:58

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes

Published on: March 22, 2022

4.2K
Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

16.5K

Related Experiment Videos

Last Updated: Sep 3, 2025

Comparative Analysis of Automatic Fecal Analyzer versus Direct Wet Smear Microscopy for Detecting Parasitic Infections in Stool Samples
04:57

Comparative Analysis of Automatic Fecal Analyzer versus Direct Wet Smear Microscopy for Detecting Parasitic Infections in Stool Samples

Published on: April 25, 2025

579
Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
05:58

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes

Published on: March 22, 2022

4.2K
Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

16.5K

Area of Science:

  • Malariology
  • Molecular Biology
  • Entomology

Background:

  • The study evaluates a PCR method targeting the Plasmodium vivax mitochondrial cytochrome oxidase I (COX-I) gene against the established Circumsporozoite Protein (CSP) ELISA.
  • The comparison focuses on identifying Plasmodium vivax life stages within the Anopheles arabiensis mosquito vector.

Discussion:

  • The authors identified questionable agreement using Cohen's kappa statistic between the mt COX-I PCR and CSP ELISA methods.
  • This highlights potential limitations in the statistical validation of diagnostic methods for Plasmodium vivax detection.

Key Insights:

  • The reliability of diagnostic tools in parasitology depends heavily on appropriate statistical validation.
  • The study suggests that the chosen statistical method may not accurately reflect the concordance between the two diagnostic approaches.

Outlook:

  • Further research should employ robust statistical methodologies to validate novel diagnostic techniques for Plasmodium vivax.
  • Accurate identification of malaria parasites in vectors is crucial for effective vector control strategies and disease transmission interruption.