Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same authorSame journal

Optimising Haematopoietic Stem Cell Transplantation: Enhancing Myeloablation Sensitivity and Alleviating Anaemia Using Roxadustat (FG-4592).

Cell proliferation·2026
Same author

Integrin-mediated mechanotransduction in the tumor microenvironment: macrophage-centered signaling mechanisms and immune remodeling.

Journal of translational medicine·2026
Same author

Inhba is transcriptionally regulated by Foxa1 through the PI3K/AKT signal pathway in acute lung injury.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Comment on "Prevalence and Correlates of Sleep Problems in Children and Adolescents with Type 1 Diabetes: A Cross-Sectional Study" [Letter].

Diabetes, metabolic syndrome and obesity : targets and therapy·2026
Same author

[Effectiveness Analysis of Cervical Rotation-Traction Manipulation on Cervical and Dorsal Muscle Tone in Patients With Cervical Spondylotic Radiculopathy].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition·2026
Same author

Predictive Accuracy of Intraocular Lens Power Calculation Formulas for Cataract Surgery in Keratoconus: A Systematic Review and Network Meta-Analysis [Letter].

Clinical ophthalmology (Auckland, N.Z.)·2026

Related Experiment Video

Updated: Sep 3, 2025

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens
09:33

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

1.2K

Spatial transcriptomic profiling to identify mesoderm progenitors with precision genomic screening and functional

Guanghui Liu1, Guanheng Yang1, Guijun Zhao1

  • 1Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Cell Proliferation
|July 30, 2022
PubMed
Summary

This study identifies 50 candidate mesoderm-specific genes crucial for development using advanced sequencing and bioinformatics. These findings offer new insights into mesoderm progenitor identification and function.

More Related Videos

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

11.6K

Related Experiment Videos

Last Updated: Sep 3, 2025

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens
09:33

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

1.2K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

11.6K

Area of Science:

  • Developmental Biology
  • Genomics
  • Molecular Biology

Background:

  • Mesoderm development is critical for forming diverse tissues and organs.
  • Systematic identification of mesoderm-specific genes and markers remains a significant challenge in developmental biology.
  • Understanding mesoderm development is key to regenerative medicine and disease research.

Purpose of the Study:

  • To screen and identify novel candidate genes essential for mesoderm development.
  • To elucidate the specific characteristics and markers of mesodermal cells.
  • To validate the identified genes through functional assays.

Main Methods:

  • Laser capture microdissection and microcellular RNA sequencing of cells from three germ layers.
  • Bioinformatic analysis to identify mesoderm-specific differentially expressed genes (DEGs).
  • Validation using real-time quantitative polymerase chain reaction, immunohistochemistry, ESCs-EBs differentiation, and colony-forming units (CFUs) assays.

Main Results:

  • Identified 1962 differentially expressed mesoderm genes, with 50 selected as candidate mesoderm-specific DEGs.
  • Gene Ontology (GO) analysis revealed these genes are involved in somite development, germ layer formation, segmentation, and pattern specification.
  • Key genes (Cdh2, Cdh11, Jag1, T, Fn-1, Pcdh7) showed specific mesodermal expression; Pcdh7 demonstrated hematopoietic functions.

Conclusions:

  • Spatial transcriptomic profiling combined with multi-method analysis successfully identified candidate mesoderm progenitors.
  • The employed approach is efficient and reliable for screening and validating candidate genes.
  • This methodology can be extended to diverse cellular systems for gene discovery in developmental processes.