Activity of Essential Oils Against Multidrug-Resistant Salmonella enteritidis
Sana Alibi1, Walid Ben Selma2,3,4, Hedi Ben Mansour2,3
1Research Unit Analysis and Process Applied to the Environment UR17ES32, Higher Institute of Applied Sciences and Technology, Rejiche Street, 5121, Mahdia, Tunisia. alibi_sana@hotmail.fr.
Current Microbiology
|July 30, 2022
Summary
Six essential oils (EOs) demonstrate potent antibacterial and anti-biofilm activity against multidrug-resistant Salmonella enteritidis. Clove, thyme, and cinnamon EOs show significant anti-quorum sensing and anti-virulence effects.
Area of Science:
- Microbiology
- Natural Products Chemistry
- Infectious Diseases
Background:
- Multidrug-resistant (MDR) Salmonella enteritidis poses a significant threat to public health.
- Essential oils (EOs) are natural compounds with potential antimicrobial properties.
- Bacterial biofilm formation and quorum sensing (QS) contribute to antibiotic resistance and virulence.
Purpose of the Study:
- To evaluate the antibacterial, anti-biofilm, and anti-quorum sensing activities of six EOs against MDR S. enteritidis.
- To assess the efficacy of EOs in reducing bacterial adhesion and virulence in a model organism.
Main Methods:
- Microdilution method for determining Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC).
- Biofilm inhibition assay on polystyrene plates.
- Quorum sensing inhibition assay using Chromobacterium violaceum CV026.
- Assessment of bacterial adhesion to HT-29 cells and virulence in Caenorhabditis elegans.
Main Results:
- Cinnamon, thyme, and clove EOs exhibited strong antibacterial properties against MDR S. enteritidis.
- All six EOs significantly reduced biofilm formation, with cinnamon, clove, and thyme showing >95% inhibition.
- Clove, thyme, and cinnamon EOs effectively inhibited quorum sensing, with clove EO showing 99.03% inhibition.
- Thyme EOs demonstrated significant anti-adhesive properties, and clove EO reduced Salmonella virulence in C. elegans.
Conclusions:
- Essential oils from cinnamon, thyme, and clove possess significant potential as natural agents against MDR S. enteritidis.
- These EOs can combat bacterial infections by inhibiting biofilm formation, quorum sensing, adhesion, and virulence.
- Further research into these EOs could lead to novel therapeutic strategies for combating antibiotic-resistant bacterial infections.
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