Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

What is dental caries - and why we need fluoride.

Caries research·2026
Same author

In silico study of hyposalivation and sugar exposure on biofilm dysbiosis.

JADA foundational science·2026
Same author

Cell-Free Protein Synthesis in Porous Parylene Scaffolds.

Small methods·2026
Same author

Minimum Intervention Oral Care (MIOC) - Overcoming Implementation Barriers: An International Expert Consensus.

International dental journal·2026
Same author

Surface-Capped Protein Nanoparticles for Nonviral Gene Delivery.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Targeting of Bacteria Using Amylase-Degradable, Copper-Loaded Starch Nanoparticles.

Antibiotics (Basel, Switzerland)·2026

Related Experiment Video

Updated: Sep 3, 2025

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique
05:42

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique

Published on: July 27, 2022

3.7K

Early occlusal caries detection using targeted fluorescent starch nanoparticles.

Nathan A Jones1, Wendy Bloembergen1, Livia M A Tenuta2

  • 1GreenMark Biomedical Inc., 1600 Huron Parkway Building 520, Room 2399, Ann Arbor, MI 48109, United States.

Journal of Dentistry
|July 30, 2022
PubMed
Summary

Fluorescent cationic starch nanoparticles (FCSNs) accurately detect occlusal caries in vitro. This novel technology shows high sensitivity and specificity, aiding early detection and promoting non-invasive dental treatments.

More Related Videos

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
11:09

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci

Published on: January 25, 2011

17.9K
Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
09:44

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Published on: October 20, 2011

16.2K

Related Experiment Videos

Last Updated: Sep 3, 2025

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique
05:42

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique

Published on: July 27, 2022

3.7K
An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
11:09

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci

Published on: January 25, 2011

17.9K
Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
09:44

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Published on: October 20, 2011

16.2K

Area of Science:

  • Biomaterials Science
  • Dental Diagnostics
  • Nanotechnology

Background:

  • Early detection of occlusal caries is crucial for conservative treatment.
  • Current diagnostic methods for occlusal caries have limitations.
  • Fluorescent cationic starch nanoparticles (FCSNs) have shown potential for enamel caries detection.

Purpose of the Study:

  • To evaluate the in vitro diagnostic accuracy of FCSNs in detecting occlusal caries.
  • To compare the performance of FCSNs against a histologic reference standard.
  • To assess the reproducibility of FCSN-based caries detection.

Main Methods:

  • 100 extracted human teeth with varying caries severity were analyzed.
  • Regions of interest were assessed for fluorescence after FCSN application.
  • Histology served as the gold standard for caries classification.
  • Sensitivity, specificity, and ROC AUC were calculated.

Main Results:

  • FCSNs demonstrated high diagnostic accuracy for occlusal caries (ROC AUC = 0.92).
  • Substantial inter-examiner (k=0.74) and high intra-examiner agreement (k=0.80, 0.94) were observed.
  • Sensitivity and specificity were 88.9% and 94.6% for all lesions, respectively.

Conclusions:

  • FCSNs represent a reproducible and accurate novel technology for occlusal caries detection.
  • The technology shows promise for improving early caries detection, potentially leading to more conservative treatments.
  • Further clinical validation is required to confirm these findings in a real-world setting.