How to build a tumor: An industry perspective

Area of Science:

• Biomedical Engineering
• Cell Biology
• Drug Discovery

Background:

• Over 15 years, numerous 3D in vitro systems have emerged, enabling isolated manipulation of cellular and molecular disease contributors.
• These advanced models offer potential for identifying key factors in disease pathogenesis.

Purpose of the Study:

• To review prominent 3D in vitro systems, focusing on their applications and translational relevance.
• To provide an industry perspective on the drivers for adopting these advanced models.
• To aid researchers in selecting appropriate 3D models by detailing their pros and cons.

Main Methods:

• Literature review of innovative 3D in vitro systems.
• Analysis of system applications, translational relevance, and industry adoption factors.
• Comparative assessment of different 3D model platforms.

Main Results:

• A wide array of 3D in vitro systems are available, offering enhanced control over experimental variables.
• Key challenges remain in improving the predictivity and validity of current 3D models.
• Selecting the optimal assay and readout combination is critical for addressing specific scientific inquiries.

Conclusions:

• A thorough understanding of 3D in vitro platforms is essential for effective model selection.
• Continued development is needed to enhance the predictive power and translational value of 3D models.
• This review serves as a guide for researchers and industry professionals navigating the landscape of 3D in vitro systems.