Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Sep 2, 2025

Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks

Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks

Published on: November 2, 2020

Bone tissue engineering supported by bioprinted cell constructs with endothelial cell spheroids.

WonJin Kim¹, Chul Ho Jang², GeunHyung Kim^1,3

¹Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 16419, South Korea.

|August 1, 2022

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Three-Finger Borescope-Mounted IPMC Gripper.

Soft robotics·2026

Same author

Auditory and Tinnitus Outcomes of Vibrant Soundbridge Implantation with the Incus Short Process Coupler in Older Male Veterans.

Brain sciences·2026

Same author

Tailorable porous collagen hydrogels as a physiologically relevant platform for extrachromosomal DNA-associated colorectal cancer research.

Theranostics·2026

Same author

Shape-Memory Collagen/Silk-Fibroin Scaffold for Dura Sealing and Skull Base Regeneration.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Mastoid Obliteration After Canal Wall Down Mastoidectomy Using Tissue Engineering Approaches with Polymers, Mesenchymal Stem Cells, and Bioactive Molecules: A Systematic Review.

Bioengineering (Basel, Switzerland)·2026

Same author

Comparing Slim Straight and Slim Perimodiolar Electrode Arrays for Cochlear Implantation: Hearing Results and Risks-A Systematic Review (2015-2025).

Audiology research·2026

This study introduces a novel hybrid structure using endothelial cell (EC) spheroids and human adipose stem cells (hASCs) for enhanced bone regeneration. The innovative approach significantly improves vascularization and bone formation in vivo.

Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Vascularized bone tissue formation is critical for successful bone regeneration.
Current strategies often face challenges in achieving efficient vascularization and osteogenesis simultaneously.

Purpose of the Study:

To develop and evaluate a novel hybrid cell construct for enhanced bone tissue engineering.
To investigate the synergistic effects of endothelial cell (EC) spheroids and human adipose stem cells (hASCs) in a bioprinted structure.

Main Methods:

Fabrication of EC spheroids using cell-mixed mineral oil.
Interlayering EC spheroids within bioprinted hASC-laden struts.
In vitro evaluation of cellular responses and gene expression.

Keywords:

Tissue engineering bioprinting bone multiple cells spheroids

More Related Videos

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

Related Experiment Videos

Last Updated: Sep 2, 2025

Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks

Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks

Published on: November 2, 2020

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

In vivo assessment of bone formation in a rat mastoid model.

Main Results:

Spheroid-laden constructs demonstrated superior angiogenesis and osteogenic activity compared to conventionally bioprinted constructs.
EC spheroids facilitated enhanced crosstalk between ECs and hASCs, boosting osteogenic and angiogenic gene expression.
Significantly greater new bone formation and vascularization were observed in vivo.

Conclusions:

The bioprinted spheroid-laden multiple-cell construct shows significant potential for bone tissue engineering applications.
This strategy effectively leverages EC spheroids to promote vascularization and osteogenesis.
The findings support the use of this hybrid construct for challenging bone regeneration scenarios.