Demonstration of locally synthesized borrelia antibodies in cerebrospinal fluid

Antibodies against Borrelia burgdorferi develop slowly. Low titers can be anamnestic. In order to prove the etiology of manifestations of erythema chronicum migrans disease of the nervous system like meningopolyneuritis Garin-Bujadoux-Bannwarth or progressive borrelia encephalomyelitis we used the ELISA to measure specific IgG antibodies against Ixodes ricinus borrelia per microgram IgG in serum and cerebrospinal fluid. With the mentioned method we were able to demonstrate locally synthesized antibodies in the cerebrospinal fluid. Until the 110th day of illness a difference between serum and CSF in favour of the latter as high as five binary dilution steps could be found. Later on in time the difference decreased to four dilution steps or less.