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LncRNA STK4 antisense RNA 1 (STK4-AS1) promoted osteosarcoma by inhibiting p53 expression

Weitao Yao1, Jingyu Hou1, Guoqing Liu1

  • 1Affiliated Tumor Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, China.

Cancer Biomarkers : Section a of Disease Markers
|August 1, 2022
PubMed
Abstract

Insights

Long non-coding RNA STK4 antisense RNA 1 (STK4-AS1) promotes osteosarcoma cell proliferation by inhibiting p53 expression, impacting cell cycle progression. This finding highlights STK4-AS1 as a potential therapeutic target for osteosarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNA STK4 antisense RNA 1 (STK4-AS1) is implicated as a potential biomarker in various cancers.
  • This study investigates the role of STK4-AS1 in osteosarcoma proliferation, specifically its regulation of the cell cycle.

Purpose of the Study:

  • To determine the expression levels of STK4-AS1, p53, and p21 in osteosarcoma compared to normal tissues.
  • To elucidate the functional role of STK4-AS1 in regulating osteosarcoma cell cycle progression and viability.
  • To explore the relationship between STK4-AS1 and the p53/p21 pathway in osteosarcoma.

Main Methods:

  • Comparative analysis of STK4-AS1, p53, and p21 expression in clinical osteosarcoma tissues and cell lines.
  • Functional assays involving overexpression and knockdown of STK4-AS1 in osteosarcoma (U2OS, MG63) and normal osteoblast (hFOB) cell lines.
  • Cell cycle analysis, cell viability assays, and Western blotting to assess protein expression (p53, p21, cyclins).

Main Results:

  • STK4-AS1 expression was elevated, while p53 and p21 expression was reduced in osteosarcoma samples.
  • STK4-AS1 knockdown in U2OS cells decreased viability, arrested the cell cycle at G0/G1, and increased p53/p21 levels.
  • Functional effects of STK4-AS1 were dependent on p53 expression, with MG63 cells (p53-null) showing no significant changes.

Conclusions:

  • LncRNA STK4-AS1 promotes osteosarcoma cell cycle progression by inhibiting p53 expression.
  • STK4-AS1 acts as an oncogenic lncRNA in osteosarcoma, potentially through the p53/p21 pathway.
  • Targeting STK4-AS1 may offer a novel therapeutic strategy for osteosarcoma.
Keywords:
STK4-AS1cell cycleosteosarcomap53 protein

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