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Updated: Sep 2, 2025

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Chemical control over the conversion between bicyclic and polycyclic terpenes by fungal bifunctional terpene
Xinye Wang1, Zhixin Wang1, Guoliang Zhu1
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. chenq@ecust.edu.cn.
Abstract:
Fungal bifunctional terpene synthases (BFTSs) reportedly associate with a series of new skeletons of di/sesterterpenes. However, the molecular mechanisms underlying the variabilities in the ring system of BFTS-catalyzed products are not well understood. In this study, we identified a key site, S89/L89, that controls the conversion between bicyclic and polycyclic terpene skeletons catalyzed by two BFTSs, BsPS and FoFS. Our analysis revealed that a mutation on site 89 in the BFTSs alters the carbocation transportation pathway and redirects the competing reactions for previously unreported terpenes.
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