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Measurement of Chitinase Activity in Biological Samples
Published on: August 22, 2019
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Chitinase 3-Like 2
Vidya P Nimbalkar1, Banavathy S Kruthika1, Palavalasa Sravya1
1Department of Neuropathology, National Institute of Mental Health and Neurosciences, Bangalore, India.
American Journal of Clinical Pathology
|August 1, 2022
Summary
High expression of chitinase 3-like 2 (CHI3L2) in glioblastoma (GBM) correlates with tumor recurrence and poorer patient survival. This suggests CHI3L2 may drive tumor spread and recurrence in GBM patients.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Cancer Research
Background:
- Glioblastoma (GBM) is an aggressive brain tumor with poor prognosis.
- Understanding molecular markers associated with GBM progression and recurrence is crucial for improving patient outcomes.
Purpose of the Study:
- To investigate the expression pattern of chitinase 3-like 2 (CHI3L2) in primary and recurrent glioblastoma.
- To determine the association between CHI3L2 expression in the tumor core and peritumoral brain zone (PBZ) and patient prognosis.
Main Methods:
- Quantitative polymerase chain reaction (qPCR) and immunohistochemistry (IHC) were used to analyze CHI3L2 mRNA and protein expression.
- Three independent patient cohorts were utilized, including paired primary-recurrent GBM tissues and a The Cancer Genome Atlas (TCGA) cohort.
Main Results:
- CHI3L2 mRNA expression was significantly elevated in GBM tumor tissues (core and PBZ) compared to controls.
- Recurrent GBM tumors exhibited higher CHI3L2 expression than primary tumors.
- High CHI3L2 expression was significantly associated with shorter overall survival and progression-free survival.
Conclusions:
- Elevated CHI3L2 expression in GBM, particularly in recurrent tumors, is linked to unfavorable patient prognosis.
- CHI3L2 may play a role in promoting glioblastoma tumor spread and recurrence.
