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Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...
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Related Experiment Video

Updated: Sep 2, 2025

Whole-Kidney Three-Dimensional Staining with CUBIC
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Whole-Kidney Three-Dimensional Staining with CUBIC.

Sho Hasegawa1, Masaomi Nangaku2

  • 1Division of Nephrology and Endocrinology, The University of Tokyo; hasesho.jpn@gmail.com.

Journal of Visualized Experiments : Jove
|August 1, 2022
PubMed
Summary

This study introduces a new whole-mount mouse kidney staining protocol using CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis) tissue clearing. This method enables detailed 3D imaging of kidney structures, aiding in understanding kidney diseases.

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Area of Science:

  • Nephrology
  • Biomedical Imaging
  • Histology

Background:

  • Conventional kidney pathology lacks 3D structural detail for microvasculature, tubules, glomeruli, and nerves.
  • Existing whole-organ imaging techniques struggle with antibody penetration for effective cell labeling.

Purpose of the Study:

  • To develop a robust whole-mount mouse kidney staining protocol for 3D imaging.
  • To overcome limitations in antibody penetration for whole-organ analysis.

Main Methods:

  • Utilized the CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis) tissue clearing method.
  • Developed a whole-mount staining protocol specifically optimized for mouse kidneys.
  • Combined tissue clearing with 3D imaging techniques for single-cell resolution analysis.

Main Results:

  • Successfully visualized renal sympathetic denervation following ischemia-reperfusion injury in 3D.
  • Enabled the detection of glomerulomegaly in early-stage diabetic kidney disease.
  • Demonstrated the capability to analyze kidney structures from a macroscopic perspective.

Conclusions:

  • The developed protocol provides unprecedented 3D insights into kidney microstructure.
  • This technique facilitates new discoveries in kidney research by offering a comprehensive macroscopic viewpoint.
  • It is a valuable tool for studying kidney physiology and pathology.