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Related Concept Videos

Cell Migration01:09

Cell Migration

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Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
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Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
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Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
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Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

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Human CD99L2 Regulates a Unique Step in Leukocyte Transmigration.

Nakisha S Rutledge1, Faith T Ogungbe1, Richard L Watson1

  • 1Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL.

Journal of Immunology (Baltimore, Md. : 1950)
|August 1, 2022
PubMed
Summary
This summary is machine-generated.

CD99-like 2 (CD99L2) is crucial for leukocyte migration during inflammation. This study reveals its novel role in human cell transmigration, distinct from PECAM and CD99.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD99-like 2 (CD99L2) is a type 1 membrane protein vital for leukocyte transendothelial migration (TEM) in mice.
  • Previous studies focused on CD99L2's role in murine inflammation, leaving its function in human inflammation and TEM mechanisms unknown.

Purpose of the Study:

  • To investigate the role of CD99L2 in human inflammation and elucidate its mechanism in leukocyte TEM.
  • To determine if CD99L2 functions similarly to PECAM and CD99 in human endothelial cells and leukocytes.

Main Methods:

  • Studied CD99L2 expression on primary human endothelial cells and leukocytes.
  • Utilized antibody blockade and genetic knockdown to inhibit CD99L2 function.
  • Assessed the impact of CD99L2 inhibition on human neutrophil and monocyte transmigration across endothelial cells.
  • Investigated the sequential steps of TEM involving CD99L2, PECAM, and CD99.

Main Results:

  • Human CD99L2 is constitutively expressed at endothelial cell borders and on leukocytes, similar to PECAM and CD99.
  • Inhibiting CD99L2 significantly reduced human neutrophil and monocyte transmigration.
  • CD99L2 regulates a specific step in TEM, acting sequentially between PECAM and CD99.
  • CD99L2 facilitates TEM by recruiting the lateral border recycling compartment, downstream of PECAM.

Conclusions:

  • Identified a novel functional role for human CD99L2 in leukocyte TEM.
  • Elucidated a TEM mechanism for CD99L2 that is distinct from PECAM and CD99.
  • CD99L2 represents a potential therapeutic target for inflammatory diseases.