Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.5K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.5K
Computed Tomography01:10

Computed Tomography

5.7K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
5.7K
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

44
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
44
Positron Emission Tomography01:29

Positron Emission Tomography

5.1K
Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
5.1K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

263
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
263

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Superconducting phase diagram of multilayer square-planar nickelates.

Science (New York, N.Y.)·2026
Same author

Imaging of a van der Waals spin-orbit torque system using spin ensembles in hBN.

Nature communications·2026
Same author

Engineering <i>Escherichia coli</i> for Urease-Driven Synthesis of Metal Oxide Nanomaterials.

ACS synthetic biology·2026
Same author

Chiral Microneedle Arrays With Terahertz Chiroptical Activity With Chiral-Plasmon-Chiral-Phonon Resonance.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Characterization Standard for <i>In-situ</i> Cryo-electron Tomography.

bioRxiv : the preprint server for biology·2026
Same author

Atomic-Scale Imaging of Lithium Vacancies in a Battery Cathode by Multislice Electron Ptychography.

Nano letters·2026
Same journal

Interplay between oxygen redox and interfacial stability of Li-rich positive electrodes in sulfide-based all-solid-state batteries.

Nature communications·2026
Same journal

Breaking dependence on melanisation imparts diversity to a dogmatic invasion strategy of phytopathogenic fungi.

Nature communications·2026
Same journal

Hydroxyl-rich nanocavities on perovskite enable nearly barrierless intramolecular hydrogen transfer for nitrate electroreduction to ammonia.

Nature communications·2026
Same journal

Household mobility responses to weather extremes in Kyrgyzstan.

Nature communications·2026
Same journal

Autonomous Motion Vision with Tri-bulk-heterojunctioned Organic Adaptation Transistor.

Nature communications·2026
Same journal

Tissue-adhesive hydrogel optical fiber for peripheral optogenetic neuromodulation.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Sep 2, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

14.2K

Real-time 3D analysis during electron tomography using tomviz.

Jonathan Schwartz1, Chris Harris2, Jacob Pietryga1

  • 1Department of Material Science and Engineering, University of Michigan, Ann Arbor, MI, USA.

Nature Communications
|August 1, 2022
PubMed
Summary
This summary is machine-generated.

Real-time electron tomography accelerates 3D imaging for biological and material sciences. This new method provides rapid 3D tomogram visualization, enabling faster specimen analysis directly during electron microscopy experiments.

More Related Videos

Electron Cryotomography of Bacterial Cells
14:23

Electron Cryotomography of Bacterial Cells

Published on: May 6, 2010

25.6K
Leveraging Virtual Reality for Immersive Segmentation and Analysis of Cryo-Electron Tomography Data
07:17

Leveraging Virtual Reality for Immersive Segmentation and Analysis of Cryo-Electron Tomography Data

Published on: January 24, 2025

1.0K

Related Experiment Videos

Last Updated: Sep 2, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

14.2K
Electron Cryotomography of Bacterial Cells
14:23

Electron Cryotomography of Bacterial Cells

Published on: May 6, 2010

25.6K
Leveraging Virtual Reality for Immersive Segmentation and Analysis of Cryo-Electron Tomography Data
07:17

Leveraging Virtual Reality for Immersive Segmentation and Analysis of Cryo-Electron Tomography Data

Published on: January 24, 2025

1.0K

Area of Science:

  • Materials Science
  • Biophysics
  • Microscopy

Background:

  • High-throughput electron tomography is crucial for 3D imaging in various scientific fields.
  • Current alignment and reconstruction processes are time-consuming, creating computational bottlenecks.
  • Rapid interpretation of specimen structure is often limited by these lengthy processing times.

Purpose of the Study:

  • To develop and demonstrate a real-time electron tomography system for immediate 3D data visualization.
  • To significantly reduce the time required for obtaining high-quality tomograms.
  • To enhance the accessibility of 3D structural analysis for scientists.

Main Methods:

  • Implementation of real-time tomography with dynamic 3D visualization capabilities.
  • Integration of the system into tomviz, an open-source, cross-platform 3D data analysis tool with a GUI.
  • Testing with geometrically complex chiral nanoparticles to assess performance.

Main Results:

  • Volumetric interpretation of complex structures became possible in under 10 minutes.
  • High-quality tomograms were generated within 30 minutes.
  • The system enables immediate interpretation of specimen structure as data is acquired.

Conclusions:

  • Real-time tomography overcomes computational bottlenecks in electron tomography.
  • The integrated tomviz tool makes advanced 3D structural characterization accessible to a wider scientific audience.
  • This approach significantly accelerates the analysis of biological and material structures using electron microscopy.