Related Experiment Video
Updated: Sep 2, 2025
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Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
Published on: August 21, 2020
12.6K
Tissue response to different incision tools in animal model.
Renato Torres-Augusto Neto1, Cássio Amaro Comachio2, Lilian Caldas Quirino de Almeida2
1School of Dentistry, Araraquara - Diagnosis and Surgery Department, São Paulo State University (UNESP), Rua Humaitá, 1680 - Centro - Araraquara, Araraquara, SP, 14801-903, Brazil. rtorresneto@gmail.com.
Oral and Maxillofacial Surgery
|August 1, 2022
Summary
The microdissection tip and conventional scalpel blade show superior tissue repair compared to other methods. The microdissection tip offers effective incision and hemostatic control without compromising healing.
Area of Science:
- Surgical techniques
- Histology
- Tissue repair
Background:
- Evaluating novel surgical tools is crucial for improving patient outcomes.
- Understanding the comparative efficacy of different incision methods is essential in surgical practice.
Purpose of the Study:
- To compare the tissue repair efficacy of microdissection electrocautery tip, conventional electrocautery tip, high potency diode laser, and conventional scalpel blade.
- To assess the histomorphometric differences in wound healing across various time points.
Main Methods:
- Incisions were made on Holtzman rats using four distinct instruments.
- Animals were euthanized at 24 hours, 48 hours, 72 hours, 7 days, and 14 days post-incision.
- Quantitative histological and histomorphometric analyses were conducted using HE and Picrosirius Red staining.
Main Results:
- Minimal statistically significant differences were observed in inflammatory profiles and tissue repair between the conventional scalpel blade and the microdissection tip.
- Both the conventional scalpel blade and microdissection tip demonstrated quantitatively superior tissue repair compared to the electrocautery and diode laser methods.
- The microdissection tip provided effective hemostasis comparable to conventional electrocautery without negatively impacting tissue repair.
Conclusions:
- The microdissection tip is effective for creating incisions, comparable to a conventional scalpel blade, but with enhanced efficacy.
- This technique offers improved hemostatic control in the surgical field, similar to conventional electrocautery, while maintaining tissue repair integrity.
- The findings suggest the microdissection electrocautery tip as a promising alternative for surgical incisions, balancing efficacy and tissue healing.
