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Updated: Sep 2, 2025
Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
Published on: August 21, 2020
Tissue response to different incision tools in animal model
Renato Torres-Augusto Neto1, Cássio Amaro Comachio2, Lilian Caldas Quirino de Almeida2
1School of Dentistry, Araraquara - Diagnosis and Surgery Department, São Paulo State University (UNESP), Rua Humaitá, 1680 - Centro - Araraquara, Araraquara, SP, 14801-903, Brazil. rtorresneto@gmail.com.
Purpose:
The aim of this study is to compare the repair of incisions performed with microdissection electrocautery tip, conventional electrocautery tip, high potency diode laser, and conventional scalpel blade in a in vivo model.
Methods:
Different incisions were performed in adults Holtzman rats using the four types of instruments: microdissection electrocautery tip, conventional electrocautery tip, high potency diode laser, and conventional scalpel blade, in different periods of healing process. Thirty rats were divided into 5 groups, according to the period of euthanasia-24 h, 48 h, 72 h, 7 days, and 14 days. All animals received four incisions, each by a different method. Quantitative histological and histomorphometric analyses were performed using hematoxylin and eosin (HE) and Picrosirius Red staining.
Results:
Inflammatory profile and tissue repair presented small statistically significance differences comparing conventional scalpel blade and microdissection tip; moreover, both presented quantitatively superior to the others.
Conclusion:
It is believed that the microdissection tip can perform a dynamic incision just as a common scalpel blade, but more effective. Furthermore, it can promote a better hemostatic control of the surgical field that is comparable to conventional electrocautery tip without affecting tissue repair.
