Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

iPS Cell Differentiation01:22

iPS Cell Differentiation

2.8K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DCLK1-dependent NF-κB activation mediates p-STAT3-induced osteoarthritis progression.

Clinical and translational medicine·2026
Same author

Risk Factors for Sarcopenia in Women with Type 2 Diabetes and the Effects of Metformin: A Cross-Sectional Study of 7,731 Patients from the UK Biobank.

International journal of women's health·2025
Same author

Association Between Sarcopenia and the Long-Term Risk of Intervertebral Disc Degeneration.

Journal of cachexia, sarcopenia and muscle·2025
Same author

Understanding the role of NOTCH2 mutation in centronuclear myopathy.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Correction: Deep learning model for automated detection of fresh and old vertebral fractures on thoracolumbar CT.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2025
Same author

Corrigendum to "IL-32 aggravates metabolic disturbance in human nucleus pulposus cells by activating FAT4-mediated Hippo/YAP signaling" [Int. Immunopharmacol. 141 (2024) 112966].

International immunopharmacology·2024

Related Experiment Video

Updated: Sep 2, 2025

Operational and Intervention Effects of Targeted Tuina in Lumbar Intervertebral Disc Degeneration Model Rabbits
06:05

Operational and Intervention Effects of Targeted Tuina in Lumbar Intervertebral Disc Degeneration Model Rabbits

Published on: July 21, 2023

606

Irisin Ameliorates Intervertebral Disc Degeneration by Activating LATS/YAP/CTGF Signaling.

Taiqiu Chen1, Youxi Lin1, Zizhao Wu2

  • 1Department of Orthopedics, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.

Oxidative Medicine and Cellular Longevity
|August 2, 2022
PubMed
Summary

Irisin, derived from FNDC5, shows protective effects against intervertebral disc degeneration (IDD) by restoring extracellular matrix metabolism in nucleus pulposus cells (NPCs) through the LATS/YAP/CTGF pathway.

More Related Videos

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.
05:46

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

Published on: February 14, 2021

3.8K
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
08:03

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Published on: April 11, 2017

9.7K

Related Experiment Videos

Last Updated: Sep 2, 2025

Operational and Intervention Effects of Targeted Tuina in Lumbar Intervertebral Disc Degeneration Model Rabbits
06:05

Operational and Intervention Effects of Targeted Tuina in Lumbar Intervertebral Disc Degeneration Model Rabbits

Published on: July 21, 2023

606
A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.
05:46

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

Published on: February 14, 2021

3.8K
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
08:03

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Published on: April 11, 2017

9.7K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Regenerative Medicine

Background:

  • Intervertebral disc degeneration (IDD) is linked to imbalanced extracellular matrix (ECM) metabolism in nucleus pulposus cells (NPCs).
  • Irisin, a myokine from fibronectin type III domain-containing 5 (FNDC5), regulates ECM metabolism, but its role in IDD is unclear.

Purpose of the Study:

  • To investigate the protective effects and molecular mechanisms of irisin in intervertebral disc degeneration (IDD).
  • To explore irisin's impact on nucleus pulposus cells (NPCs) and ECM metabolism.

Main Methods:

  • Analysis of FNDC5, COL2A1, ACAN, and ADAMTS4 expression in degenerative NP tissues.
  • In vivo study using a rat IDD model with irisin treatment.
  • In vitro study using TNF-α-stimulated NPCs treated with irisin.
  • RNA sequencing to identify signaling pathways affected by irisin.
  • Investigation of the Hippo signaling pathway (LATS, YAP) and CTGF involvement.

Main Results:

  • Degenerative NP tissues showed decreased FNDC5 and anabolic markers (COL2A1, ACAN) with increased catabolic markers (ADAMTS4).
  • Irisin treatment slowed IDD progression in rats and reversed ECM metabolic disorder in stimulated NPCs.
  • Irisin modulated the Hippo pathway, downregulating LATS/YAP phosphorylation and upregulating CTGF.
  • CTGF knockdown partially abolished irisin's protective effects on NPC ECM metabolism.

Conclusions:

  • Irisin treatment promotes ECM anabolism and inhibits catabolism in NPCs, delaying IDD progression.
  • The protective effects of irisin involve the LATS/YAP/CTGF signaling pathway.
  • Irisin represents a potential therapeutic target for treating intervertebral disc degeneration.