A fish-scale derived multifunctional nanofiber membrane for infected wound healing

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Nanotechnology

Background:

• Modern medicine requires advanced wound healing solutions for infections.
• Existing dressings face challenges in providing multifunctional wound treatment.
• Fish scale collagen offers a promising biomaterial for wound dressings.

Purpose of the Study:

• To develop a multifunctional nanofibrous membrane for infected wound healing.
• To evaluate the efficacy of fish scale collagen-based nanofibers with copper sulfide nanoparticles (CuS) and near-infrared (NIR) light.
• To assess the impact on hemostasis, inflammation, cell proliferation, and tissue remodeling.

Main Methods:

• Preparation of a nanofibrous membrane using electrospun fish scale collagen (FSC), polyvinylpyrrolidone (PVP), polyvinyl butyral (PVB), and NIR-adsorbed CuS nanoparticles (FSC/CuS).
• Evaluation of wound healing parameters including hemostasis time, inflammatory response, cell proliferation, and tissue remodeling.
• Histological analysis using Hematoxylin and Eosin (H&E), Masson staining, and immunohistochemical staining.

Main Results:

• The FSC/CuS nanofibrous membrane combined with NIR light demonstrated synergistic effects in wound healing.
• Inhibition of inflammatory response and promotion of fibroblast and keratinocyte proliferation and migration were observed.
• Enhanced tissue rebuilding, formation of well-dispersed collagen fibers, and accelerated healing across all evaluated stages were achieved.

Conclusions:

• The developed FSC/CuS nanofibrous scaffold shows excellent performance in accelerating infected wound healing.
• The combination of fish scale-derived collagen, NIR-absorbing agents, and NIR light offers a promising strategy for advanced wound care.
• This approach has broad application prospects for treating infected wounds.