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Updated: Sep 2, 2025

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
Published on: April 28, 2014
pH-Dependent fluorescence of [La(OH)2]+[ARS]- hybrid nanoparticles for intracellular pH-sensing
Kristina Sabljo1, Joanna Napp2,3, Frauke Alves2,3
1Karlsruhe Institute of Technology (KIT), Institute for Inorganic Chemistry, Engesserstrasse 15, 76131, Karlsruhe, Germany. claus.feldmann@kit.edu.
Abstract:
Saline inorganic-organic hybrid nanoparticles (IOH-NPs) [La(OH)2]+[ARS]- (ARS: alizarin red S) are prepared in water as a new compound (particle size: 47 ± 7 nm, ARS load: 65 wt%). The IOH-NPs not only show a pH-dependent absorption colour but also a pH-dependent fluorescence with green emission at pH 5.0-9.0 and red emission at pH < 4.5. According to first in vitro studies, the pH-dependend fluorescence can be used to monitor nanoparticle internalization in cells as well as the respective intracellular pH.
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