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Updated: Sep 2, 2025
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
Published on: September 7, 2022
Study on aptamer based high throughput approach identifies natural ingredients against RGNNV
Hongling Wei1, Mingzhu Liu1, Ke Ke1
1Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Academy of Sciences, Nanning, China.
Nervous necrosis virus (NNV) causes significant losses in marine fish aquaculture. This study introduces an aptamer-based high-throughput screening (AHTS) model for rapidly identifying natural compounds effective against NNV.
Area of Science:
- Aquaculture
- Virology
- Biotechnology
Background:
- Nervous necrosis virus (NNV) is a major pathogen in marine fish aquaculture, causing substantial economic losses worldwide.
- Current methods for detecting NNV and evaluating antiviral efficacy, like RT-qPCR, are costly and time-consuming.
- Aptamers offer a promising alternative for diagnostics and therapeutics due to their specificity, stability, and low cost.
Purpose of the Study:
- To establish an aptamer (GBN34)-based high-throughput screening (GBN34-AHTS) model.
- To enable efficient selection and evaluation of natural ingredients against NNV infection.
- To provide a rapid, sensitive, and accurate method for identifying antiviral compounds.
Main Methods:
- Development of a GBN34 aptamer.
- Establishment of a high-throughput screening (HTS) platform utilizing the GBN34 aptamer.
- Application of the GBN34-AHTS model to screen natural ingredients for anti-NNV activity.
Main Results:
- The GBN34-AHTS model demonstrated high-speed, sensitive, and accurate screening capabilities.
- The AHTS strategy significantly reduced experimental costs and shortened the screening cycle.
- The model proved effective in identifying natural ingredients with potential anti-NNV properties.
Conclusions:
- The GBN34-AHTS model is an efficient and cost-effective tool for selecting natural antiviral compounds against NNV.
- This rapid screening approach can accelerate the discovery of treatments for NNV and potentially other viral diseases in aquaculture.
- The study highlights the potential of aptamer-based HTS in combating aquatic viral pathogens and improving aquaculture sustainability.
