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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Three-Dimensional Microscopy in Microbiology01:28

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Related Experiment Video

Updated: Sep 2, 2025

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
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Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

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Computational methods for three-dimensional electron microscopy (3DEM)

Jose-Jesus Fernandez1, A Martinez-Sanchez2

  • 1Spanish National Research Council (CINN-CSIC) and Health Research Institute of Asturias (ISPA), Av Hospital Universitario s/n, Oviedo 33011, Spain.

Computer Methods and Programs in Biomedicine
|August 2, 2022
PubMed
Summary

No abstract available in PubMed .

Keywords:
3DEMComputational methodsThree-dimensional electron microscopy

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