Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

52.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.8K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

182
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
182
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative analysis of <i>Asterias forbesi</i> development reveals distinct mechanisms of hydro-vascular organ formation across sea stars.

Discover developmental biology·2026
Same author

Trans-Phylum Single Cell Orthology Reveals Conserved Ovarian Cell States Between Sea Urchin and Human.

Genome biology and evolution·2026
Same author

Single cell RNA seq of the major cell types in the larva of the sea star, Patiria miniata.

Developmental dynamics : an official publication of the American Association of Anatomists·2025
Same author

Collagen processing is essential for germ cell identity.

Biology open·2025
Same author

Conservation and diversity of genes expressed in the spines of the sea urchin.

Genes & genomics·2025
Same author

A collagenous extracellular matrix regulates germline gene expression in the sea star embryo.

Scientific reports·2025

Related Experiment Video

Updated: Sep 2, 2025

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.2K

Optimizing CRISPR/Cas9-based gene manipulation in echinoderms.

Nathalie Oulhen1, Cosmo Pieplow1, Margherita Perillo1

  • 1MCB Department, Brown University, Providence, RI, 02906, USA.

Developmental Biology
|August 2, 2022
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas9 technology offers a more effective way for researchers to study sea urchins and sea stars. Standardized protocols and education can accelerate the adoption of these powerful gene-editing tools in echinoderm research.

Keywords:
Cas9MicroinjectionMorpholino antisense oligonucleotides (MASO)Sea starSea urchinSynthetic single guide RNAs

More Related Videos

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.7K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.3K

Related Experiment Videos

Last Updated: Sep 2, 2025

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.2K
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.7K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.3K

Area of Science:

  • Marine Biology
  • Developmental Biology
  • Genetics

Background:

  • Traditional methods like pharmacology and morpholino antisense oligonucleotide (MASO) technologies have limitations in studying model organisms.
  • CRISPR-Cas9 gene editing offers enhanced efficiency and broader applicability for biological research.

Purpose of the Study:

  • To optimize CRISPR-Cas9 technology for applications in echinoderms (sea urchin and sea star).
  • To provide standardized protocols and guidance for setting up and interpreting CRISPR-Cas9 experiments in these species.
  • To lower the barrier for broader laboratory adoption of CRISPR-Cas9 in echinoderm research.

Main Methods:

  • Optimization of CRISPR-Cas9 protocols specifically for sea urchin and sea star models.
  • Development of guidelines for experimental design and result interpretation.
  • Compilation of successful case examples from other research groups.

Main Results:

  • Successfully optimized CRISPR-Cas9 gene editing in sea urchin and sea star.
  • Established practical advice for researchers new to CRISPR-Cas9 in echinoderms.
  • Demonstrated the potential for effective gene function studies using CRISPR-Cas9 in these marine invertebrates.

Conclusions:

  • CRISPR-Cas9 technology significantly enhances the study of echinoderms.
  • Standardized protocols and shared knowledge are crucial for advancing CRISPR-Cas9 applications.
  • Increased accessibility of CRISPR-Cas9 will accelerate discoveries in marine biology and developmental genetics.