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Related Experiment Video

Updated: Sep 2, 2025

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Precise epigenomic editing with a SunTag-based modular epigenetic toolkit.

Subhrangshu Guhathakurta1, Levi Adams1,2, Inhye Jeong2

  • 1Burnett School of Biomedical Sciences, UCF College of Medicine, University of Central Florida, Orlando, FL USA.

Epigenetics
|August 3, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a CRISPR/dCas9 toolkit for precise epigenetic gene regulation. This system modifies specific histone marks to understand their impact on gene expression with high resolution.

Keywords:
CRISPREpigeneticsepigenetic writersepigenome editinghistone post-translational modifications

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Epigenetic modifications are key regulators of gene expression.
  • Understanding the precise role of individual epigenetic marks is challenging.
  • CRISPR/dCas9 technology offers potential for targeted epigenetic manipulation.

Purpose of the Study:

  • To develop a modular CRISPR/dCas9-based toolkit for gene-specific epigenetic modulation.
  • To enable the targeted modification of multiple epigenetic marks.
  • To investigate the functional impact of specific histone modifications on gene expression.

Main Methods:

  • Engineered a CRISPR/dCas9 system using the SunTag framework with GCN4 moieties.
  • Fused epigenetic writers to scFv and directed them using target-specific sgRNAs.
  • Demonstrated modification of individual histone post-translational modifications.

Main Results:

  • Successfully modulated specific epigenetic marks in a target-specific manner.
  • Showcased the toolkit's efficiency in altering individual histone modifications.
  • Screened promoter regions to identify differential gene expression outcomes with base-pair resolution.

Conclusions:

  • The developed toolkit provides a versatile platform for gene-specific epigenetic engineering.
  • It facilitates the study of gene-specific epigenetic changes and their link to gene expression.
  • The system is adaptable for various epigenetic effectors, aiding research in epigenetics.