Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

4.6K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-resolution single-cell mapping of clonal hematopoiesis and structural variation in aplastic anemia.

Nature genetics·2026
Same author

<i>ARID5B</i> influences B-cell development and function in mouse.

Haematologica·2023
Same author

Genomics of deletion 7 and 7q in myeloid neoplasm: from pathogenic culprits to potential synthetic lethal therapeutic targets.

Leukemia·2023
Same author

Molecular Mechanisms of ARID5B-Mediated Genetic Susceptibility to Acute Lymphoblastic Leukemia.

Journal of the National Cancer Institute·2022
Same author

Publisher Correction: Clinical evolution, genetic landscape and trajectories of clonal hematopoiesis in SAMD9/SAMD9L syndromes.

Nature medicine·2021
Same author

Gain-of-function mutations in RPA1 cause a syndrome with short telomeres and somatic genetic rescue.

Blood·2021
Same journal

Emapalumab and eltrombopag rescue for prolonged immune effector cell-associated hemophagocytic syndrome after CAR T-cell therapy in multiple myeloma.

Haematologica·2026
Same journal

Incidence and predictors of recurrent venous thromboembolism after isolated distal deep vein thrombosis: a <i>post-hoc</i> analysis of the RIDTS trial.

Haematologica·2026
Same journal

Philadelphia positive T-acute lymphoblastic leukemia: a Mayo Clinic series.

Haematologica·2026
Same journal

Long-term follow-up of oral decitabine/cedazuridine plus venetoclax for older or unfit patients with newly diagnosed acute myeloid leukemia.

Haematologica·2026
Same journal

Reframing leukemia-niche interactions: the emerging role of the marrow adipocyte lineage.

Haematologica·2026
Same journal

A novel dose-dense strategy for CD19-directed CAR T-cell therapy is associated with durable responses without increased toxicity in patients with B-cell non-Hodgkin lymphoma.

Haematologica·2026
See all related articles

Related Experiment Video

Updated: Sep 2, 2025

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
08:22

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Published on: May 31, 2020

5.2K

ARID5B influences B-cell development and function in mouse.

Charnise Goodings1, Xujie Zhao1, Shannon McKinney-Freeman2

  • 1Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, TN.

Haematologica
|August 4, 2022
PubMed
Summary
This summary is machine-generated.

The ARID5B gene, linked to childhood leukemia risk, plays a crucial role in B-cell development. Overexpressing Arid5b in mice impaired B-cell maturation and function, suggesting its importance in hematopoiesis.

More Related Videos

Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

16.5K
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

6.2K

Related Experiment Videos

Last Updated: Sep 2, 2025

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
08:22

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Published on: May 31, 2020

5.2K
Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

16.5K
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

6.2K

Area of Science:

  • Immunology
  • Genetics
  • Hematology

Background:

  • Childhood acute lymphoblastic leukemia (ALL) susceptibility has a known inherited basis.
  • Genome-wide association studies identified risk variants at the ARID5B gene locus.
  • The precise molecular mechanisms of ARID5B in normal and malignant hematopoiesis are largely unknown.

Purpose of the Study:

  • To investigate the in vivo role of Arid5b in hematopoiesis using a transgenic mouse model.
  • To elucidate the molecular mechanisms by which ARID5B influences B-cell development and function.

Main Methods:

  • Utilized a Vav1-driven transgenic mouse model to overexpress Arid5b.
  • Analyzed B-cell populations in peripheral blood, bone marrow, and spleen.
  • Assessed B-cell activation and B-cell receptor signaling in vitro.
  • Measured mitochondrial oxygen consumption rates in B cells.

Main Results:

  • Arid5b overexpression significantly reduced circulating B cells, including immature and mature fractions, and splenic follicular B cells.
  • In vitro studies revealed defects in B-cell activation with hyperactivated B-cell receptor signaling.
  • Arid5b-overexpressing B cells exhibited increased mitochondrial oxygen consumption rates.

Conclusions:

  • ARID5B plays a critical role in normal B-cell development and function.
  • Dysregulation of ARID5B may contribute to hematological malignancies like ALL.
  • Further research into ARID5B's mechanisms could reveal therapeutic targets for leukemia.