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Published on: July 3, 2015
Linear dose response of acrocentric chromosome associations to gamma irradiation in human lymphocytes
Ravindra M Samarth1, Puneet Gandhi2, Nabo Kumar Chaudhury3
1Department of Research, ICMR-Bhopal Memorial Hospital & Research Centre (ICMR-BMHRC), Government of India, Raisen Bypass Road, 462038, Bhopal, India. rmsamarth@gmail.com.
Purpose:
The frequency of acrocentric chromosome associations (ACA) was studied to determine the possible dose-response relationship of gamma irradiation in human lymphocytes.
Methods:
Peripheral blood collected from three healthy donors was irradiated with 0, 0.1, 0.25, 0.5, 0.75, 1, 2, 3, 4, and 5 Gy of gamma radiation. Chromosomal preparations were made after 48 h of culture as per standard guidelines. The experiment was repeated three times, with a different donor each time.
Results:
The ACA frequency in irradiated lymphocytes increased with radiation dose. The D-G type of association was most prominent and showed a significant dose-dependent increase in frequency. The dose response of ACA frequency to radiation was found to be linear: ACA frequency = 0.2923 (±0.0276) + 0.1846 (±0.0307) × D (correlation coefficient r = 0.9442). As expected, dicentric chromosome (DC) frequencies followed the linear quadratic fit model, with DC frequency = 0.0015 (±0.0013) + 0.0220 (±0.0059) × D + 0.0215 (±0.0018) × D^2 (correlation coefficient r = 0.9982). A correlation curve was prepared for ACA frequency versus DC frequency, resulting in the regression equation y = 1.130x + 0.4051 (R2 = 0.7408; p = 0.0014).
Conclusion:
Our results showed an increase in ACA frequency in irradiated lymphocytes with an increase in radiation dose; thus, ACA may serve as a candidate cytogenetic biomarker for radiation biodosimetry.
