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An improved extraction procedure for an HPLC method for cyclosporin A in whole blood and plasma
Annals of Clinical Biochemistry
|March 1, 1987
Abstract:
Cyclosporin A (CyA) was assayed by high performance liquid chromatography (HPLC) following extraction of the whole blood or plasma with diethyl ether and chromatography on a Sep-Pak Silica cartridge. Using carefully chosen volumes and ratios of ethyl acetate and hexane in the eluting sequences on the Sep-Pak, the major interferent present in normal blood and serum was eliminated.