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Related Experiment Video

Updated: Sep 2, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

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Practical Considerations for Single-Cell Genomics.

Claire Regan1, Jonathan Preall1

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

Current Protocols
|August 4, 2022
PubMed
Summary

The single-cell genomics revolution offers powerful tools for analyzing RNA, DNA, and proteins at the individual cell level. This guide provides fundamental principles for study design, sample preparation, and quality control in single-cell genomics experiments.

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Multi-site Assessment of Methods for Cell Preservation Upstream of Single Cell RNA Sequencing.

bioRxiv : the preprint server for biology·2025

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • The field of genomics is rapidly advancing with new single-cell technologies.
  • These technologies enable the analysis of RNA expression, DNA alterations, protein abundance, and chromatin accessibility at single-cell resolution.
  • The increasing complexity of these techniques necessitates guidance for researchers.

Purpose of the Study:

  • To provide an overview of popular single-cell genomics technologies.
  • To address common questions regarding experimental design, sample preparation, quality control, and sequencing strategies.
  • To highlight fundamental principles applicable to most single-cell genomics workflows.

Main Methods:

  • Review of current single-cell genomics technologies, with a focus on single-cell RNA sequencing (scRNA-seq).
Keywords:
genomicsscATAC-seqscRNA-seqsequencingsingle-celltranscriptomics

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  • Discussion of essential considerations for experimental design and sample handling.
  • Explanation of quality control metrics and sequencing strategies relevant to single-cell data.

    • Main Results:

    • Identification of key principles for successful single-cell genomics experiments.
    • Emphasis on the importance of careful planning and execution to avoid common pitfalls.
    • Highlighting the versatility and growing applications of single-cell genomics.

    Conclusions:

    • Single-cell genomics technologies are transformative tools for biological research.
    • Adherence to fundamental principles is crucial for maximizing the advantages of these platforms.
    • This overview serves as a practical guide for researchers navigating the landscape of single-cell genomics.