Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.9K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.9K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

990
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
990
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

5.7K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.7K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

7.0K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
7.0K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial Expression of Concern: Recognition of the nonclassical MHC class I molecule H2-M3 by the receptor Ly49A regulates the licensing and activation of NK cells.

Nature immunology·2026
Same author

Optimization Leading to a Potent and Selective Cbl‑b Inactive-State Inhibitor That Demonstrated <i>In Vivo</i> Efficacy.

ACS medicinal chemistry letters·2026
Same author

B cells enable autoreactive T cells to avoid suppression.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

IL-6R blockade with tocilizumab disrupts pericyte- and tumor cell-driven IL-6/STAT3 signaling, enhancing docetaxel efficacy in ER+ breast cancer.

bioRxiv : the preprint server for biology·2026
Same author

Post-COVID impairment of memory T cell responses to community-acquired pathogens can be rectified by activating cellular metabolism.

bioRxiv : the preprint server for biology·2026
Same author

Transcription factor Zfx regulates tumor's evasion to T cell killing in immunotherapy.

iScience·2025

Related Experiment Video

Updated: Sep 2, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.8K

METTL14-dependent m6A modification controls iNKT cell development and function.

Liang Cao1, Eva Morgun1, Samantha Genardi1

  • 1Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, 320 E. Superior Street, Searle 3-401, Chicago, IL 60611, USA.

Cell Reports
|August 4, 2022
PubMed
Summary
This summary is machine-generated.

N6-methyladenosine (m6A) RNA modification is crucial for invariant natural killer T (iNKT) cell development and function. Loss of METTL14 impairs iNKT cell homeostasis, leading to reduced numbers and function.

Keywords:
CD1CP: ImmunologyCP: Molecular biologyNKT cellsT cell developmentknockout micem(6)A

More Related Videos

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

8.8K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K

Related Experiment Videos

Last Updated: Sep 2, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.8K
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

8.8K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K

Area of Science:

  • Immunology
  • Epigenetics
  • Molecular Biology

Background:

  • N6-methyladenosine (m6A) is the most prevalent RNA modification, regulating gene expression.
  • m6A controls CD4+ T cell homeostasis via the IL-7/STAT5/SOCS pathway.
  • The function of m6A in unconventional T cell development is largely unexplored.

Purpose of the Study:

  • To investigate the role of m6A modification, specifically mediated by METTL14, in the development and homeostasis of invariant natural killer T (iNKT) cells.

Main Methods:

  • Utilized mice with T cell-specific deletion of the RNA methyltransferase METTL14 (T-Mettl14-/-).
  • Analyzed thymocyte apoptosis, Vα14-Jα18 gene rearrangement, and iNKT cell populations in vivo.
  • Assessed iNKT cell maturation, responsiveness to cytokine and TCR stimulation, and cytokine production following METTL14 knockdown.

Main Results:

  • T-Mettl14-/- mice exhibited increased thymocyte apoptosis and reduced Vα14-Jα18 rearrangements, leading to significantly fewer iNKT cells.
  • Residual iNKT cells in T-Mettl14-/- mice showed enhanced apoptosis, impaired maturation, and diminished responsiveness to IL-2/IL-15 and TCR signaling.
  • METTL14 knockdown in mature iNKT cells decreased cytokine production, associated with increased Cish expression and impaired TCR signaling.

Conclusions:

  • METTL14-dependent m6A modification is essential for iNKT cell development, homeostasis, and function.
  • Loss of m6A modification disrupts iNKT cell generation and survival by affecting thymocyte apoptosis and TCR signaling pathways.
  • This study establishes m6A RNA modification as a critical regulator of unconventional T cell biology.