Response mechanisms of microalgal-bacterial granular sludge to zinc oxide nanoparticles

Currently, zinc oxide nanoparticles (ZnO-NPs) with their widespread applications lead to their increasing dosages in wastewater, posing an urgent threat to wastewater treatment. Herein, the responses of the emerging microalgal-bacterial granular sludge (MBGS) to ZnO-NPs were investigated. The results showed that the performance of MBGS was significantly affected when the concentration of ZnO-NPs reached 10 mg/L, especially for the removal of ammonia and phosphorus. ZnO-NPs on the granular surface could affect microalgae photosynthesis by shading, while antioxidant enzymes could be generated against overproduced reactive oxygen species. Specifically, ZnO-NPs addition to MBGS systems altered the microbial community structure (e.g. Cyanobacteria) and function (e.g. biosynthesis) of prokaryotes rather than eukaryotes. Overall, the MBGS could exhibit multiple mechanisms to alleviate the ZnO-NPs toxicity. This study is expected to add knowledge on MBGS in the treatment of wastewater containing nanoparticles.