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Updated: Sep 2, 2025

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction
Published on: July 26, 2024
Structural characterization of a water-soluble and antimicrobial β-glucan secreted by Rhizobium pusense
Bin Zhang1, Zhenna Zhang1, Xiaomei Lyu2
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Abstract:
In this study, a bacterium with the ability to extracellularly produce a water-soluble polysaccharide (with high molecular mass of 743 kDa) was obtained from saline soils. This strain named as ZB01 was identified as Rhizobium punsense by 16S rRNA sequence analysis. The monomer composition and structure of extracellular polysaccharides were investigated by high-performance anion-exchange chromatography-pulsed amperometric detector, Fourier-transform infrared, methylation and nuclear magnetic resonance spectroscopy analysis. In addition, the morphological characterization of extracellular polysaccharides was performed by scanning electron microscopy analysis. As a result, the polysaccharide is found to be a novel water-soluble type of glucan most likely consisting of repeating two β-d-Glcp-(1 → 3) and one α-d-Glcp-(1 → 3). Furthermore, it showed pronounced antimicrobial effects against Escherichia coli and Bacillus subtilis, suggesting it has the potential to be used as a natural antibacterial agent in the future.
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