Related Experiment Video
Updated: Sep 2, 2025

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
Published on: May 28, 2021
Cathodoluminescence imaging of cellular structures labeled with luminescent iridium or rhenium complexes at cryogenic
Marie Vancová1,2, Radim Skoupý3, Eva Ďurinová4,5
1Institute of Parasitology, Biology Centre CAS, České Budějovice, 37005, Czech Republic. vancova@paru.cas.cz.
Abstract:
We report for the first time the use of two live-cell imaging agents from the group of luminescent transition metal complexes (IRAZOLVE-MITO and REZOLVE-ER) as cathodoluminescent probes. This first experimental demonstration shows the application of both probes for the identification of cellular structures at the nanoscale and near the native state directly in the cryo-scanning electron microscope. This approach can potentially be applied to correlative and multimodal approaches and used to target specific regions within vitrified samples at low electron beam energies.
More Related Videos
Related Concept Videos
Total Internal Reflection Fluorescence Microscopy
Photoluminescence: Applications
Three-Dimensional Microscopy in Microbiology
Cryo-electron Microscopy

