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Updated: Sep 2, 2025

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Long Non-coding RNA UCA1a Promotes Proliferation via PKM2 in Cervical Cancer.

Minmin Yu1,2, Songlin Xue3, Xin Chen3

  • 1Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, China. njyy022@njucm.edu.cn.

Reproductive Sciences (Thousand Oaks, Calif.)
|August 4, 2022
PubMed
Summary

The long non-coding RNA Urothelial Cancer-Associated 1a (UCA1a) promotes cervical cancer progression by interacting with Pyruvate Kinase M2 (PKM2). UCA1a regulates PKM2 stability and nuclear translocation, driving HeLa cell proliferation.

Keywords:
Cervical cancerPKM2UCA1a

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cervical cancer is a significant global health concern for women.
  • The long non-coding RNA Urothelial Cancer-Associated 1a (UCA1a) is upregulated in cervical cancer, but its function is unclear.

Purpose of the Study:

  • To elucidate the role and mechanism of UCA1a in cervical cancer progression.
  • To identify key regulatory regions and interacting proteins of UCA1a.

Main Methods:

  • RNA sequencing to analyze gene expression changes in HeLa cells overexpressing UCA1a.
  • Mass spectrometry to identify UCA1a-interacting proteins.
  • Functional assays to assess the impact of UCA1a and PKM2 on cell proliferation.

Main Results:

  • Overexpression of UCA1a significantly altered gene expression profiles in HeLa cells, particularly affecting tumorigenesis pathways.
  • Pyruvate Kinase M2 (PKM2) was identified as a UCA1a-interacting protein, with specific regions critical for binding.
  • UCA1a promotes HeLa cell proliferation by stabilizing PKM2 and regulating its nuclear translocation, involving ubiquitination degradation pathways.

Conclusions:

  • UCA1a plays a crucial role in cervical cancer progression.
  • A novel mechanism involving UCA1a-mediated regulation of PKM2 stability and function in HeLa cells was identified.
  • UCA1a represents a potential therapeutic target for cervical cancer.