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Related Experiment Videos

Fluorescence from pilosebaceous follicles.

A Johnsson, B Kjeldstad, T B Melø

    Archives of Dermatological Research
    |January 1, 1987
    PubMed
    Summary

    Fluorescence analysis of pilosebaceous follicle extrusions revealed porphyrins produced by Propionibacterium acnes (P. acnes). These findings suggest P. acnes as a source of fluorescence in skin follicles.

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    Area of Science:

    • Dermatology
    • Microbiology
    • Biophysics

    Background:

    • Pilosebaceous follicles are integral to skin health and can be affected by microbial activity.
    • Propionibacterium acnes (P. acnes) is a bacterium commonly found in the skin microbiome.
    • Porphyrins are fluorescent molecules produced by various organisms, including bacteria.

    Purpose of the Study:

    • To investigate the fluorescence properties of extrusions from pilosebaceous follicles.
    • To identify the source of fluorescence observed in follicle samples.
    • To characterize the porphyrins present in these samples.

    Main Methods:

    • Fluorescence spectroscopy was employed on follicle extrusions obtained via pressure extraction.
    • Samples were analyzed using a fluorometer to obtain corrected excitation and emission spectra.
    • Spectra were compared with those of cultured P. acnes cells.

    Main Results:

    • Follicle extrusions exhibited fluorescence under Wood's light, consistent with porphyrin presence.
    • Emission spectra showed distinct features, indicating the presence of at least three porphyrins.
    • Spectra closely resembled those of cultured P. acnes, supporting bacterial origin.
    • Coproporphyrins and metalloporphyrins were identified, while free protoporphyrins were not detected.

    Conclusions:

    • The fluorescence observed in pilosebaceous follicle extrusions is primarily attributed to porphyrins produced by Propionibacterium acnes (P. acnes).
    • Individual variations in porphyrin concentrations and spectral features were noted across subjects.
    • The findings provide insights into the biochemical activity of P. acnes within the pilosebaceous unit.

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