Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Septic Cardiac Remodeling: A New Concept in Cardiac Dysfunction Induced by Experimental Sepsis.

Antioxidants (Basel, Switzerland)·2026
Same author

Phosphoethanolamine: a translational journey from biological process and physiopathological effects to therapeutical innovation - a mini-review.

Frontiers in pharmacology·2026
Same author

Machine learning-enhanced plasma proteomics discriminates pancreatic cancer-associated diabetes from type 2 diabetes mellitus.

Journal of proteomics·2026
Same author

Antimicrobial Peptides and Systemic Inflammation: A Network Analysis.

Immunity, inflammation and disease·2026
Same author

Cross-talk between lipopolysaccharide tolerance and AGEs in the regulation of macrophage inflammation and cholesterol efflux.

Frontiers in immunology·2026
Same author

Nanoyeast-based impedimetric biosensor with mutated single chain antigen-binding fragment anchoring for SARS-CoV-2 detection.

Biomedical microdevices·2026

Related Experiment Video

Updated: Sep 2, 2025

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
09:41

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

13.4K

Evidence for Monocyte Reprogramming in a Long-Term Postsepsis Study.

Raquel Bragante Gritte1, Talita Souza-Siqueira1, Eliane Borges da Silva1

  • 1Interdisciplinary Post-Graduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo, SP, Brazil.

Critical Care Explorations
|August 5, 2022
PubMed
Summary
This summary is machine-generated.

Sepsis alters monocyte gene expression, with inflammatory markers remaining elevated for years post-discharge. This persistent reprogramming may contribute to long-term sepsis complications and mortality.

Keywords:
M1 macrophageM2 macrophageinflammasomepostsepsis syndrome

More Related Videos

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
09:57

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

9.8K
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
09:32

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Published on: September 9, 2014

70.1K

Related Experiment Videos

Last Updated: Sep 2, 2025

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
09:41

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

13.4K
Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
09:57

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

9.8K
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
09:32

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Published on: September 9, 2014

70.1K

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Sepsis is a life-threatening condition characterized by a dysregulated host response to infection.
  • Monocytes play a crucial role in the inflammatory response during sepsis.
  • The long-term effects of sepsis on monocyte function and their potential contribution to postsepsis syndrome are not fully understood.

Purpose of the Study:

  • To investigate persistent monocyte alterations in sepsis survivors after hospital discharge.
  • To evaluate the gene expression of inflammatory mediators and monocyte polarization markers.
  • To determine if sepsis reprograms monocytes in a manner that influences long-term patient outcomes.

Main Methods:

  • Gene expression profiling of monocytes was performed.
  • Assessed inflammatory receptors, M1/M2 polarization markers, NLRP3 inflammasome components, and pro-/anti-inflammatory cytokines.
  • Monocytes were analyzed from 34 patients at multiple time points post-sepsis (discharge, 3 months, 6 months, 1 year, 3 years) and compared to controls.

Main Results:

  • Upregulation of inflammatory receptors (TLR2, TLR4) and NLRP3 inflammasome components (NLRP3, NFκB1, caspase 1, etc.) was observed.
  • Proinflammatory cytokines (IL-1α, IL-1β, IL-18, HMGB1) and M1 polarization markers (CXCL10, CXCL11, IL-12p35) were elevated.
  • Anti-inflammatory/tissue repair markers (IL-10, CCL14, CCL22, TGF-β, SR-B1, PPARγ) also showed upregulation, indicating a complex reprogramming.

Conclusions:

  • Sepsis induces significant and persistent reprogramming of monocyte inflammatory states.
  • These long-term monocyte alterations likely contribute to the development of postsepsis syndrome.
  • Persistent monocyte reprogramming may be a key factor in sepsis-related long-term morbidity and mortality.