ARFs get the BioID treatment: what have we been missing?

Area of Science:

• Cell Biology
• Molecular Biology
• Biochemistry

Background:

• The Arf family of small GTPases are key regulators of intracellular membrane trafficking.
• Understanding the specific functions and interactions of Arf GTPases is crucial for deciphering cellular processes.

Purpose of the Study:

• To perform a proximity-interaction screen to identify effector proteins for 25 Arf family members.
• To characterize novel cellular roles for understudied Arf GTPases.

Main Methods:

• Mammalian cell culture
• Proximity-interaction screening
• Biochemical assays

Main Results:

• An important resource of Arf GTPase effector interactions was generated.
• Novel roles were identified for ARL11/14 in regulating Phospholipase D1 (PLD1) during phagocytosis.
• New functions for ARL5A/5B in regulating Phosphatidylinositol 4-kinase beta (PI4KB) in the Golgi were characterized.

Conclusions:

• This study provides a valuable resource for cell biology research.
• The findings reveal new regulatory mechanisms involving Arf GTPases in phagocytosis and Golgi homeostasis.