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In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
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Accurate In Vivo Bowman's Thickness Measurement Using Mirau Ultrahigh Axial Resolution Line Field Optical Coherence

Samuel Lawman1,2, Sharon Mason2, Stephen B Kaye2

  • 1University of Liverpool, Faculty of Science and Engineering, Department of Electrical Engineering and Electronics, Liverpool, UK.

Translational Vision Science & Technology
|August 5, 2022
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Summary

This study demonstrates that ultrahigh axial resolution (UHR) line field spectral domain (LF-SD) optical coherence tomography (OCT) accurately measures corneal layer thickness. The technology is repeatable and provides reliable reference ranges for healthy corneas.

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Area of Science:

  • Ophthalmology
  • Biomedical Optics
  • Medical Imaging

Background:

  • Accurate measurement of corneal layer thickness is crucial for diagnosing and managing various eye conditions.
  • Existing optical coherence tomography (OCT) systems have limitations in achieving the desired axial resolution for precise measurements of thin corneal layers.

Purpose of the Study:

  • To evaluate the accuracy, repeatability, and performance limits of in vivo Mirau ultrahigh axial resolution (UHR) line field spectral domain (LF-SD) optical coherence tomography (OCT).
  • To establish reference ranges for Bowman's layer and epithelial thickness in healthy corneas using the developed OCT system.

Main Methods:

  • In vivo imaging of the central cornea in healthy volunteers using a Mirau-UHR-LF-SD-OCT system.
  • The system offers an axial resolution of 2.4 µm in air (1.7 µm in tissue) and high A-scan speed (204.8 kHz).
  • Image segmentation was performed to measure Bowman's and epithelial layer thicknesses.

Main Results:

  • The Mirau-UHR-LF-SD-OCT system demonstrated high accuracy and repeatability for measuring corneal layer thicknesses.
  • Repeatability for Bowman's and epithelial thickness measurements was 0.3 µm and 1.0 µm, respectively.
  • Established 95% population ranges for healthy in vivo corneal layers: Bowman's (13.7–19.6 µm) and epithelium (41.9–61.8 µm).

Conclusions:

  • The Mirau-UHR-LF-SD-OCT system provides accurate and repeatable measurements of Bowman's layer and corneal epithelial thickness.
  • The established reference ranges align with previous studies using different OCT and confocal microscopy systems.
  • This UHR OCT technology holds potential for clinical applications in corneal diagnostics.