A systematic review on potential microbial carbohydrases: current and future perspectives.

Area of Science:

• Biotechnology and Biochemistry
• Enzymology
• Microbial Technology

Background:

• Microbial proteins exhibit superior stability compared to plant and animal sources, driving interest in microbial enzymes.
• Novel microbes from extreme environments are increasingly sought for industrial enzyme applications.
• Carbohydrases are a key group of industrially significant enzymes involved in sugar production.

Approach:

• This review highlights five crucial carbohydrases: amylase, cellulase, laccase, pectinase, and xylanase.
• It details their properties, mechanisms, applications, and production optimization strategies.
• Molecular approaches for enhanced production and biotechnological perspectives are also discussed.

Key Points:

• Focus on amylase, cellulase, laccase, pectinase, and xylanase properties and mechanisms.
• Exploration of production optimization, purification, and molecular enhancement techniques.
• Discussion of green catalytic conversion using enzyme nanoparticles and future biotechnological development.

Conclusions:

• Microbial carbohydrases are vital for numerous industrial applications, including bioenergy and bioconversion.
• Sustainable strategies and advanced molecular techniques are crucial for optimizing enzyme production and stability.
• This review provides insights into the importance and future potential of carbohydrases for researchers.