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Related Experiment Video

Updated: Sep 2, 2025

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices
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Functional human cell-based vascularised cardiac tissue model for biomedical research and testing.

Maria Koivisto1, Tuomas A Tolvanen2, Tarja Toimela2

  • 1Faculty of Medicine and Health Technology, Tampere University, Arvo Ylpön katu 34, 33520, Tampere, Finland. maria.koivisto@tuni.fi.

Scientific Reports
|August 5, 2022
PubMed
Summary

This study developed a novel human cell-based cardiac tissue model using co-cultured cardiomyocytes and vascular networks. This advanced model accurately predicts drug cardiotoxicity, improving in vitro testing relevance for human settings.

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Area of Science:

  • Biomedical Engineering
  • Stem Cell Biology
  • Cardiovascular Research

Background:

  • Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are vital for in vitro research but lack full maturation.
  • Achieving adult cardiomyocyte characteristics is crucial for physiologically relevant preclinical testing.

Purpose of the Study:

  • To enhance the maturity and physiological relevance of hiPSC-CMs.
  • To develop a functional, human cell-based cardiac tissue model for improved in vitro testing.
  • To validate the model's predictive accuracy for cardiotoxicity.

Main Methods:

  • Co-culturing hiPSC-CMs with preconstructed vascular-like networks to form cardiac tissue.
  • Analyzing morphology and gene expression for maturation assessment.
  • Evaluating drug effects using multielectrode array and comparing with human clinical data.

Main Results:

  • The co-cultured cardiac tissue model demonstrated advanced maturation compared to monocultures.
  • The model achieved high predictive accuracy (91%), sensitivity (90%), and specificity (100%) for cardiac effects of 32 compounds.
  • A strong correlation (R²=0.905) was observed between in vitro effective concentrations and clinical plasma concentrations.

Conclusions:

  • The developed cardiac tissue model mimics human cardiac tissue, enabling accurate in vitro to in vivo data transfer.
  • This standardized model is highly valuable for biomedical research and cardiotoxicity testing.
  • The model enhances the physiological relevance and predictive power of preclinical drug safety assessments.