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Physical effort-induced decrease in apparent whole blood Hb-oxygen affinity normalized for intraerythrocytic pH (pHe)

A L Pawlak, E Szydłowski

    Biomedica Biochimica Acta
    |January 1, 1987
    PubMed
    Summary
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    Physical effort affects hemoglobin-oxygen affinity, influenced by pH gradients within red blood cells. Changes in these gradients correlate significantly with altered oxygen affinity and hemoglobin content after exercise.

    Area of Science:

    • Physiology
    • Biochemistry
    • Hematology

    Background:

    • Hemoglobin's oxygen affinity is crucial for oxygen transport.
    • Physical exertion can alter blood parameters, including pH and hemoglobin function.
    • Understanding these changes is vital for assessing physiological responses to exercise.

    Purpose of the Study:

    • To investigate the impact of physical effort on hemoglobin-oxygen affinity.
    • To explore the relationship between pH gradients and hemoglobin-oxygen affinity changes post-exercise.
    • To identify potential mechanisms influencing these physiological responses.

    Main Methods:

    • Studied whole blood from 28 healthy males performing physical effort.
    • Measured pH gradients between plasma and erythrocytes.

    Related Experiment Videos

  • Assessed apparent hemoglobin-oxygen affinity, normalized for intraerythrocytic pH (pHe).
  • Correlated exercise-induced changes in affinity and pH gradients.

    • Main Results:

    • A decrease in the pH gradient after exercise correlated with reduced hemoglobin-oxygen affinity.
    • An increase in the pH gradient was associated with enhanced hemoglobin-oxygen affinity and blood hemoglobin content.
    • A significant negative correlation (r=-0.66, p<0.001) was found between exercise-induced changes in Hb-oxygen affinity and pH gradient.
    • No significant differences in maximal oxygen utilization were observed between groups with varying Hb-oxygen affinity changes.

    Conclusions:

    • Exercise-induced changes in hemoglobin-oxygen affinity are closely linked to alterations in the pH gradient between plasma and erythrocytes.
    • Increased pH gradients may involve the release of older erythrocytes from the spleen.
    • Decreased parameters might be associated with lower pre-exercise intraerythrocytic pH.
    • Maximal oxygen uptake is not directly dependent on these exercise-induced shifts in Hb-oxygen affinity.