The S. cerevisiae m6A-reader Pho92 promotes timely meiotic recombination by controlling key methylated transcripts
Jérémy Scutenaire1,2,3,4, Damien Plassard1,2,3,4, Mélody Matelot1,2,3,4
1Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), 67400 Illkirch, France.
Nucleic Acids Research
|August 7, 2022
Summary
The budding yeast protein Pho92, an N6-Methyladenosine (m6A) reader, accelerates the down-regulation of specific mRNAs during meiosis. This regulation by Pho92 is crucial for timely meiotic progression and recombination.
Area of Science:
- Molecular Biology
- Genetics
- Yeast Biology
Background:
- N6-Methyladenosine (m6A) is a key mRNA modification regulating gene expression.
- m6A readers bind to modified mRNAs, influencing their fate.
- The m6A methyltransferase Ime4 and its role in meiosis are known in Saccharomyces cerevisiae.
Purpose of the Study:
- To investigate the function of the m6A reader Pho92 in budding yeast meiosis.
- To understand how m6A controls gene expression during meiotic progression.
Main Methods:
- High-throughput RNA sequencing to map Pho92-binding sites.
- UV-crosslinking to identify direct mRNA targets of Pho92.
- Analysis of Pho92 function in wild-type and mutant yeast strains.
- Site-directed mutagenesis of m6A sites in target mRNAs.
Main Results:
- Pho92 acts as an early meiotic factor promoting timely meiotic progression.
- Pho92 binds to specific mRNAs in an m6A-dependent manner during meiotic prophase.
- Pho92 promotes the down-regulation of its target mRNAs before recombination.
- Altering m6A sites in Pho92 target mRNAs delays their down-regulation and can impair meiotic progression.
Conclusions:
- Pho92 facilitates meiotic progression by accelerating mRNA down-regulation.
- m6A-dependent binding of Pho92 to mRNAs is critical for efficient meiotic recombination.
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