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Published on: October 6, 2022
A G-quadruplex/hemin structure-undamaged method to inhibit peroxidase-mimic DNAzyme activity for biosensing
Zhipeng Su1, Qian Wen1, Shiwei Li2
1Hunan Province Key Laboratory of Edible Forestry Resources Safety and Processing Utilization, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha, 410004, PR China.
Researchers developed a new method to inhibit DNAzyme activity without damaging the G-quadruplex (G4) structure. An adjacent adenine: thymine base pair effectively suppressed DNAzyme function, offering a simpler approach for biosensor development.
Area of Science:
- Biochemistry
- Molecular Biology
- Chemical Biology
Background:
- G-quadruplex (G4) DNAzymes are inhibited by damaging their structure to prevent G4/hemin complex formation.
- Existing methods require complete disruption of the G4 structure, limiting applications.
Purpose of the Study:
- To propose a novel intramolecular inhibitory effect (InE(N:N)) using adjacent base pairs to inhibit G4 DNAzyme activity.
- To investigate the mechanism of inhibition and develop new biosensing strategies.
Main Methods:
- Installation of various base pairs adjacent to the G4 core sequence.
- Investigation of the inhibitory efficiency of different base pairs.
- Mechanistic studies using spectroscopy and kinetic analysis.
- Development of InE(N:N)-based biosensors for DNA and Uracil-DNA glycosylase (UDG) detection.
Main Results:
- The adjacent adenine: thymine pair demonstrated the highest inhibitory efficiency (17-fold).
- Inhibition occurred by downregulating the formation of compound I in the catalytic cycle.
- The carbonyl group on the complementary base's hexatomic ring was crucial for inhibition.
- Two InE(N:N)-based biosensors were successfully developed for DNA and UDG detection.
Conclusions:
- The InE(N:N) strategy offers a new way to regulate G4 DNAzyme activity without structural damage.
- This approach simplifies biosensor design and enables real-time assays.
- InE(N:N) provides a promising platform for future DNAzyme applications.
