Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glycoprotein biosynthesis by human normal platelets.

P Rodríguez, O Bello, R Apitz-Castro

    Biochemical Medicine and Metabolic Biology
    |April 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    ELECLA trial: final results of perioperative chemotherapy with fluoropyrimidine and oxaliplatin in mismatch repair proficient locally advanced colon cancer.

    ESMO open·2026
    Same author

    Amlodipine use during pregnancy: Current status in the medical care field in Argentina.

    Hipertension y riesgo vascular·2025
    Same author

    [Redefining primary care: CARABELA-PC recomendations for the management of prevalent chronic diseases].

    Semergen·2025
    Same author

    Resolution of ventricular dysfunction and fibrosis in a young woman with primary hyperaldosteronism.

    Hipertension y riesgo vascular·2025
    Same author

    Sonoanatomy of the difficult airway. A case-control study.

    Revista espanola de anestesiologia y reanimacion..·2024
    Same author

    [Inter-arm blood pressure difference: Consecutive versus simultaneous measurements in treated and controlled hypertense patients].

    Hipertension y riesgo vascular·2024
    Same journal

    Time dependence of plasma malondialdehyde, oxypurines, and nucleosides during incomplete cerebral ischemia in the rat.

    Biochemical medicine and metabolic biology·1994
    Same journal

    Characteristics of proteinuria in experimental diabetes mellitus.

    Biochemical medicine and metabolic biology·1994
    Same journal

    Glutathione metabolism in Crohn's disease.

    Biochemical medicine and metabolic biology·1994
    Same journal

    Rapid antenatal diagnosis of beta-thalassemia in Chinese caused by the common 4-bp deletion in codons 41/42 using high-resolution agarose gel electrophoresis and heteroduplex detection.

    Biochemical medicine and metabolic biology·1994
    Same journal

    Hexokinase binding in ischemic and reperfused piglet brain.

    Biochemical medicine and metabolic biology·1994
    Same journal

    Effects of long-term streptozotocin diabetes on cytoskeletal and cytosolic phosphofructokinase and the levels of glucose 1,6-bisphosphate and fructose 2,6-bisphosphate in different rat muscles.

    Biochemical medicine and metabolic biology·1994
    See all related articles

    Human platelets incorporate radioactive sugars into glycoproteins. This process occurs directly via sugar nucleotide glycosylation, independent of lipid-sugar intermediates.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Hematology

    Background:

    • Understanding glycoprotein biosynthesis in human blood platelets is crucial for hematological research.
    • Previous studies have explored various pathways for sugar incorporation into platelet glycoproteins.

    Purpose of the Study:

    • To investigate the mechanism of radioactive monosaccharide incorporation into human platelet glycoproteins.
    • To determine the role of lipid-sugar intermediates in this glycosylation process.
    • To elucidate the pathway of glycoprotein biosynthesis in human platelets.

    Main Methods:

    • Incubation of washed human platelets with radioactive monosaccharides (Mannose, Galactose, Fucose, N-acetylglucosamine, N-acetylneuraminic acid).
    • Analysis of hydrolyzed labeled glycoproteins using paper chromatography.

    Related Experiment Videos

  • Assessment of the effects of bacitracin and cycloheximide on sugar incorporation.

    • Main Results:

    • Radioactive sugars were incorporated into washed human platelets and endogenous glycoproteins in a time-dependent manner.
    • Analysis confirmed the presence of glycosidic linkages and no conversion of incorporated sugars into other sugar types.
    • No evidence of lipid-sugar intermediates was found, and bacitracin did not inhibit incorporation.
    • Cycloheximide did not inhibit glycosylation, indicating sugar addition to existing proteins.

    Conclusions:

    • Glycoprotein biosynthesis in human platelets occurs via direct sugar nucleotide glycosylation.
    • Lipid-linked saccharide intermediates are not involved in this process.
    • Sugars are added to proteins already present within intact platelets.