Dual-strategy biosensing of glucose based on multifunctional CuWO₄ nanoparticles

In this research, dual-strategy biosensing of glucose was proposed based on multifunctional CuWO₄ nanoparticles (CuWO₄ NPs), which were prepared for the application of electrochemical and colorimetric sensing of glucose. CuWO₄ NPs show large specific surface area and good conductivity as well as excellent peroxidase-like activity. A sensitive and selective electrochemical glucose biosensor was fabricated with the immobilization of glucose oxidase (GOx) on a CuWO₄ NP modified electrode for enhancing the direct electron transfer behavior. A wide linear range of 0.005-1.8 mM with a low detection limit of 1.5 μM and a high sensitivity of 28.02 mA M^-1 cm^-2 were achieved by using the electrochemical biosensor. Meanwhile, a colorimetric and visual glucose biosensor was constructed based on the GOx/CuWO₄ cascade nanozyme, which shows a linear range of 0.05-1.0 mM with excellent selectivity. CuWO₄ NPs as a promising matrix open up a dual-strategy biosensor for sensitive and selective detection of glucose.