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Published on: October 26, 2017
Circulating miR-448 acts as a potential diagnostic biomarker for multiple myeloma
Dan Chen1,2, Teng Chen1, Lihong Wang3
1Department of Central Laboratory, The Affiliated Hospital of Chengde Medical College, Chengde, People's Republic of China.
Objective:
The current diagnostic methods for multiple myeloma (MM) include bone marrow aspiration and biopsy, which are not only complicated but also invasive, causing great pain to patients. Micro ribonucleotides (miRNA) exist stably in circulating plasma and could be easily detected, making them promising specific diagnostic markers for MM.
Methods:
The expression of plasma miR-448 in MM patients was detected by real-time quantitative PCR analysis. Spearman correlation was used to analyze correlations between miR-448 expression and various clinic pathological characteristics of MM patients. The ROC and the AUC (95% confidence interval) were exploited to evaluate the potential of miR-448 acting as a diagnostic marker for MM patients.
Results And Discussion:
In this study, we found that the expression level of miR-448 in patients with MM was significantly higher than that in normal people and showed statistically differential expression in different stages of MM. Besides, we observed that the abundance of miR-448 in the plasma of newly diagnosed MM patients was positively correlated with the proportion of plasma cells (PC) in the bone marrow and the concentration of serum M protein (MP), respectively. In addition, ROC analysis demonstrated the sensitivity and specificity of the diagnostic value of miR-448 for MM are 92.90% and 87.50%, respectively.
Conclusion:
Taken together, these results indicated that miR-448 may serve as a potential molecular diagnostic marker for MM.
Insights
Plasma miR-448 shows potential as a non-invasive biomarker for multiple myeloma (MM). Elevated miR-448 levels correlate with disease progression, offering a promising diagnostic tool for MM.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Current multiple myeloma (MM) diagnostics involve invasive bone marrow aspiration and biopsy.
- Micro ribonucleotides (miRNAs) are stable in plasma and suitable for non-invasive detection.
- miRNAs present a promising avenue for developing specific MM diagnostic markers.
Purpose of the Study:
- To investigate the diagnostic potential of plasma miR-448 in multiple myeloma (MM).
- To correlate miR-448 expression levels with MM clinicopathological characteristics.
Main Methods:
- Real-time quantitative PCR was used to detect plasma miR-448 expression in MM patients.
- Spearman correlation analysis assessed relationships between miR-448 and MM characteristics.
- Receiver Operating Characteristic (ROC) curve analysis evaluated diagnostic accuracy.
Main Results:
- Plasma miR-448 expression was significantly higher in MM patients compared to healthy individuals.
- miR-448 levels showed differential expression across various MM stages.
- Positive correlations were observed between plasma miR-448 and plasma cell percentage/serum M protein concentration.
- ROC analysis indicated high diagnostic sensitivity (92.90%) and specificity (87.50%) for miR-448 in MM.
Conclusions:
- Plasma miR-448 demonstrates significant potential as a non-invasive diagnostic biomarker for multiple myeloma.
- miR-448 expression levels correlate with key indicators of MM disease burden.
- Further validation of miR-448 could lead to improved MM diagnostic strategies.
