CRISPR in Modulating Antibiotic Resistance of ESKAPE Pathogens

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Ujjayani Saha1, Rashmi Gondi1, Amrita Patil1

  • 1Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Symbiosis Knowledge Village, Lavale, Pune, Maharashtra, 412115, India.

Molecular Biotechnology
|August 8, 2022
PubMed

Insights

The ESKAPE pathogens are developing antimicrobial resistance. The CRISPR-Cas system

Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • ESKAPE pathogens (Enterococcus spp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) exhibit increasing antimicrobial resistance.
  • These multidrug-resistant pathogens pose a significant threat to public health, necessitating novel therapeutic strategies.

Purpose of the Study:

  • To review the multifaceted roles of the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system in modulating antibiotic resistance within ESKAPE pathogens.
  • To elucidate the mechanisms by which CRISPR-Cas influences antibiotic resistance and biofilm formation in these critical pathogens.

Main Methods:

  • Literature review of studies investigating CRISPR-Cas systems in ESKAPE pathogens.
  • Analysis of research on the interplay between CRISPR-Cas, antibiotic resistance, and biofilm formation.

Main Results:

  • CRISPR-Cas systems can both confer and prevent antibiotic resistance in ESKAPE pathogens through diverse mechanisms.
  • The presence of CRISPR-Cas can lead to acquired antibiotic resistance in some bacterial strains.
  • CRISPR-Cas systems are linked to biofilm formation, which is often associated with increased antibiotic resistance.

Conclusions:

  • A comprehensive understanding of CRISPR-Cas system functions is crucial for developing safe and effective treatments against antibiotic-resistant ESKAPE pathogens.
  • Targeting or manipulating CRISPR-Cas systems presents a potential avenue for novel antimicrobial therapies.
Keywords:
Antibiotic resistanceBiofilmCRISPR-CasESKAPEPathogens

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