Related Experiment Video
Updated: Sep 2, 2025
Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
Published on: August 2, 2024
miRNA-23b-5p affects the proliferation, migration and invasion of osteosarcoma by targeting TMEM127
1Central Laboratory, The Central Hospital Affiliated to Shaoxing University, Shaoxing, 312030, China.
Background:
Osteosarcoma (OS) has become one of the highest mortality cancers in the world due to its late diagnosis, rapid metastasis and rapid recurrence. MicroRNAs can regulate a variety of signaling pathwas involved in cancer development, such as cell proliferation, apoptosis and migration.
Objective:
In this study, we studied the biological effects and molecular regulation of mir-23b-5p on human osteosarcoma cells.
Methods:
The proliferation of mir-23b-5p in osteosarcoma was measured by CCK8 method and EDU method. In addition, the target population was screened through the database, and the luciferase reporter gene was used to determine the association between miRNA and target gene TMEM127. We verified this result by Western blot.
Results:
We found that mir-23b-5p promotes the progression of osteosarcoma by regulating TMEM127.
Conclusions:
The results of this study show that mir-23b-5p affects the proliferation, metastasis and invasion of OS by targeting TMEM127.
Related Concept Videos
01:22MicroRNAs
02:23Abnormal Proliferation
02:30Metastasis
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...