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Updated: Sep 2, 2025

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
Published on: October 15, 2018
q value and parent energy optimization using a low-voltage ionization approach increases resolution in linear ion
Simone Madama1, Ermelinda Falletta2, Amir Mohammad Malvandi3
1I.S.B. - Ion Source & Biotechnologies srl, Milan, Italy.
Abstract:
In this work, the isolation step in the linear ion trap was performed using different "q values" conditions at a low collision-induced dissociation (CID) energy leading to the parent ion resolution improvements, reasonably due to better ion energy distribution. According to the results, we obtained a greater resolution and mass accuracy operating in both traditional electrospray and low voltage ionization near the q value = 0.778 and with a CID energy of 10%. This effect was evaluated with low-molecular-mass compounds (skatole and arginine). The proposed optimization yielded a superior instrument performance without adding technological complexity to mass spectrometry analyses.
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