Related Experiment Video

Updated: Sep 2, 2025

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
14:49

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

5.2K

The Application of the CRISPR-Cas System in Antibiotic Resistance

Shuan Tao1,2, Huimin Chen1, Na Li3

  • 1School of Medical, Jiangsu University, Zhenjiang, Jiangsu Province, 212013, People's Republic of China.

Infection and Drug Resistance
|August 9, 2022
PubMed

Insights

Antimicrobial resistance (AMR) is a global threat. The CRISPR-Cas system offers a novel gene-editing tool to combat AMR by targeting resistance genes and controlling their spread.

Area of Science:

  • Microbiology
  • Genetics
  • Biotechnology

Background:

  • Antimicrobial resistance (AMR) is a growing global health crisis, driven by the spread of resistance genes via horizontal gene transfer (HGT) on mobile genetic elements (MGEs).
  • Existing strategies to combat AMR are insufficient, necessitating novel approaches to control bacterial infections and resistance dissemination.

Purpose of the Study:

  • To review the structure, mechanism, and applications of CRISPR-Cas systems in combating antimicrobial resistance.
  • To explore the potential of CRISPR-Cas as a gene-editing tool for controlling HGT and limiting the spread of AMR.

Main Methods:

  • Review of existing literature on CRISPR-Cas systems and their role in bacterial immunity and gene editing.
  • Analysis of the mechanisms by which CRISPR-Cas targets and cleaves antibiotic resistance genes.
  • Discussion of delivery strategies for CRISPR-Cas systems in therapeutic applications.

Main Results:

  • CRISPR-Cas systems function as adaptive immune mechanisms in prokaryotes, targeting and cleaving foreign genetic material.
  • The specificity of CRISPR-Cas allows for precise targeting of DNA sequences encoding antibiotic resistance genes.
  • CRISPR-Cas technology shows promise for controlling HGT and mitigating the spread of AMR.

Conclusions:

  • CRISPR-Cas systems represent a powerful and versatile tool for developing new strategies against bacterial infections and antimicrobial resistance.
  • Further research into CRISPR-Cas delivery and application is crucial for its effective implementation in clinical settings.
  • This technology holds significant potential for controlling horizontal gene transfer and limiting the global burden of drug resistance.
Keywords:
CRISPR-Casantibiotic resistancehorizontal gene transfer

More Related Videos

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.8K
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

429

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
182
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
110
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.8K
Antibiotic Selection00:57

Antibiotic Selection

Overview
55.1K
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
186